Monitoring of short-term erythropoietin therapy in rats with acute spinal cord injury using manganese-enhanced Magnetic Resonance Imaging

• Verfasst von: Freitag, Martin T. [VerfasserIn]
• Verfasst von: Parzer, Peter [VerfasserIn]
• Verfasst von: Stieltjes, Bram [VerfasserIn]
• Titel: Monitoring of short-term erythropoietin therapy in rats with acute spinal cord injury using manganese-enhanced Magnetic Resonance Imaging
• Verf.angabe: Martin Thomas Freitag, MD, Gábor Márton, MSc, Krisztián Pajer, MSc, Jens Hartmann, MD, Nadja Walder, MD, Markus Rossmann, MD, Peter Parzer, MSc, Heinz Redl, PhD, Antal Nógrádi, MD, PhD, DSc*, Bram Stieltjes, MD, PhD*
• Jahr: 2015
• Jahr des Originals: 2014
• Umfang: 8 S.
• Fussnoten: First published: 16 December 2014 ; Gesehen am 26.06.20
• Titel Quelle: Enthalten in: Journal of neuroimaging
• Ort Quelle: Berlin [u.a.] : Wiley-Blackwell, 1991
• Jahr Quelle: 2015
• Band/Heft Quelle: 25(2015), 4, Seite 582-589
• ISSN Quelle: 1552-6569
• DOI: doi:10.1111/jon.12202
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: apoptosis
• Sach-SW: EPO
• Sach-SW: inflammation
• Sach-SW: Neuroprotection
• Sach-SW: neuroregeneration
• Sach-SW: spinal cord injury
• K10plus-PPN: 1702701662

Abstract

BACKGROUND AND PURPOSE To evaluate the short-term outcome of erythropoietin (EPO) therapy in rats with spinal cord injury (SCI) using manganese-enhanced magnetic resonance imaging (MEMRI). METHODS Rats were divided in an EPO and a control group. Laminectomy at Th11 was performed, followed by SCI. MnCl2 was applied into the cisterna magna and functional recovery was examined after injury using BBB-scoring. Then, rats were euthanized and the spinal cord was extracted for MEMRI. Finally, histological analysis was performed and correlated with MEMRI. RESULTS EPO-treated animals showed significantly better functional recovery (P = .008, r = .62) and higher mean signal-to-noise ratio (SNR) in MEMRI compared to controls for slices 10-13 (P = .017, R2 = .31) at the level of the lesion epicenter. Functional recovery correlated significantly with higher SNR values, determined using the mean SNR between slices 10 and 13 (P = .047, R2 = .36). In this region, histology revealed a significantly decreased number of microglia cells and apoptosis in EPO-treated animals. CONCLUSION MEMRI successfully depicts the therapeutic effect of EPO in early SCI that leads to a significant recovery in rats, a significantly reduced immune response and significantly reduced number of apoptotic cells at the height of the lesion epicenter.