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Verfasst von:Panić, Marko [VerfasserIn]   i
 Hata, Shoji [VerfasserIn]   i
 Neuner, Annett [VerfasserIn]   i
 Schiebel, Elmar [VerfasserIn]   i
Titel:The centrosomal linker and microtubules provide dual levels of spatial coordination of centrosomes
Verf.angabe:Marko Panic, Shoji Hata, Annett Neuner, Elmar Schiebel
E-Jahr:2015
Jahr:May 22, 2015
Umfang:22 S.
Fussnoten:Gesehen am 30.06.2020
Titel Quelle:Enthalten in: Public Library of SciencePLoS Genetics
Ort Quelle:San Francisco, Calif. : Public Library of Science, 2005
Jahr Quelle:2015
Band/Heft Quelle:11(2015,5) Artikel-Nummer e1005243, 22 Seiten
ISSN Quelle:1553-7404
Abstract:The centrosome is the principal microtubule organizing center in most animal cells. It consists of a pair of centrioles surrounded by pericentriolar material. The centrosome, like DNA, duplicates exactly once per cell cycle. During interphase duplicated centrosomes remain closely linked by a proteinaceous linker. This centrosomal linker is composed of rootletin filaments that are anchored to the centrioles via the protein C-Nap1. At the onset of mitosis the linker is dissolved by Nek2A kinase to support the formation of the bipolar mitotic spindle. The importance of the centrosomal linker for cell function during interphase awaits characterization. Here we assessed the phenotype of human RPE1 C-Nap1 knockout (KO) cells. The absence of the linker led to a modest increase in the average centrosome separation from 1 to 2.5 μm. This small impact on the degree of separation is indicative of a second level of spatial organization of centrosomes. Microtubule depolymerisation or stabilization in C-Nap1 KO cells dramatically increased the inter-centrosomal separation (> 8 μm). Thus, microtubules position centrosomes relatively close to one another in the absence of linker function. C-Nap1 KO cells had a Golgi organization defect with a two-fold expansion of the area occupied by the Golgi. When the centrosomes of C-Nap1 KO cells showed considerable separation, two spatially distinct Golgi stacks could be observed. Furthermore, migration of C-Nap1 KO cells was slower than their wild type RPE1 counterparts. These data show that the spatial organization of centrosomes is modulated by a combination of centrosomal cohesion and microtubule forces. Furthermore a modest increase in centrosome separation has major impact on Golgi organization and cell migration.
DOI:doi:10.1371/journal.pgen.1005243
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pgen.1005243
 Volltext: https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005243
 DOI: https://doi.org/10.1371/journal.pgen.1005243
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cell migration
 Centrioles
 Centrosomes
 Cloning
 DAPI staining
 Dyneins
 Microtubules
 Small interfering RNAs
K10plus-PPN:1702896676
Verknüpfungen:→ Zeitschrift

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