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Status: Bibliographieeintrag

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Verfasst von:Thomann, Stefan [VerfasserIn]   i
 Baek, Sunhwa [VerfasserIn]   i
 Ryschich, Eduard [VerfasserIn]   i
Titel:Impact of wall shear stress and ligand avidity on binding of anti-CD146-coated nanoparticles to murine tumor endothelium under flow
Verf.angabe:Stefan Thomann, Sunhwa Baek, Eduard Ryschich
E-Jahr:2015
Jahr:October 14, 2015
Umfang:9 S.
Fussnoten:Gesehen am 30.06.2020
Titel Quelle:Enthalten in: OncoTarget
Ort Quelle:[S.l.] : Impact Journals LLC, 2010
Jahr Quelle:2015
Band/Heft Quelle:6(2015), 37, Seite 39960-39968
ISSN Quelle:1949-2553
Abstract:The endothelial phenotype of tumor blood vessels differs from the liver and forms an important base for endothelium-specific targeting by antibody-coated nanoparticles. Although differences of shear stress and ligand avidity can modulate the nanoparticle binding to endothelium, these mechanisms are still poorly studied. This study analyzed the binding of antibody-coated nanoparticles to tumor and liver endothelium under controlled flow conditions and verified this binding in tumor models in vivo. Binding of anti-CD146-coated nanoparticles, but not of antibody was significantly reduced under increased wall shear stress and the degree of nanoparticle binding correlated with the avidity of the coating. The intravascular wall shear stress favors nanoparticle binding at the site of higher avidity of endothelial epitope which additionally promotes the selectivity to tumor endothelium. After intravenous application in vivo, pegylated self-coated nanoparticles showed specific binding to tumor endothelium, whereas the nanoparticle binding to the liver endothelium was very low. This study provides a rationale that selective binding of mAb-coated nanoparticles to tumor endothelium is achieved by two factors: higher expression of endothelial epitope and higher nanoparticle shearing from liver endothelium. The combination of endothelial marker targeting and the use of shear stress-controlled nanoparticle capture can be used for selective intratumoral drug delivery.
DOI:doi:10.18632/oncotarget.5662
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

DOI: https://doi.org/10.18632/oncotarget.5662
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Animals
 Antibodies, Monoclonal
 CD146 Antigen
 Drug Delivery Systems
 Endothelium, Vascular
 HCC
 Ligands
 Liver
 Liver Neoplasms, Experimental
 Mice, Transgenic
 Microscopy, Fluorescence
 nanoparticle
 Nanoparticles
 Protein Binding
 shear stress
 Stress, Mechanical
 surface avidity
 Tissue Distribution
 tumor endothelial marker
K10plus-PPN:1702933350
Verknüpfungen:→ Zeitschrift

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