| |  Online-Ressource |
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| Verfasst von: | Pausch, Patrick [VerfasserIn]  |
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| | Singh, Ujjwala [VerfasserIn] |
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| | Ahmed, Yasar Luqman [VerfasserIn] |
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| | Pillet, Benjamin [VerfasserIn] |
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| | Murat, Guillaume [VerfasserIn] |
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| | Altegoer, Florian [VerfasserIn] |
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| | Stier, Gunter [VerfasserIn] |
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| | Thoms, Matthias [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| | Sinning, Irmgard [VerfasserIn] |
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| | Bange, Gert [VerfasserIn] |
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| | Kressler, Dieter [VerfasserIn] |
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| Titel: | Co-translational capturing of nascent ribosomal proteins by their dedicated chaperones |
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| Verf.angabe: | Patrick Pausch, Ujjwala Singh, Yasar Luqman Ahmed, Benjamin Pillet, Guillaume Murat, Florian Altegoer, Gunter Stier, Matthias Thoms, Ed Hurt, Irmgard Sinning, Gert Bange & Dieter Kressler |
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| E-Jahr: | 2015 |
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| Jahr: | 26 Jun 2015 |
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| Umfang: | 15 S. |
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| Teil: | volume:6 |
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| | year:2015 |
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| | extent:15 |
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| Fussnoten: | Gesehen am 01.07.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 6(2015) Artikel-Nummer 7494, 15 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Exponentially growing yeast cells produce every minute >160,000 ribosomal proteins. Owing to their difficult physicochemical properties, the synthesis of assembly-competent ribosomal proteins represents a major challenge. Recent evidence highlights that dedicated chaperone proteins recognize the N-terminal regions of ribosomal proteins and promote their soluble expression and delivery to the assembly site. Here we explore the intuitive possibility that ribosomal proteins are captured by dedicated chaperones in a co-translational manner. Affinity purification of four chaperones (Rrb1, Syo1, Sqt1 and Yar1) selectively enriched the mRNAs encoding their specific ribosomal protein clients (Rpl3, Rpl5, Rpl10 and Rps3). X-ray crystallography reveals how the N-terminal, rRNA-binding residues of Rpl10 are shielded by Sqt1’s WD-repeat β-propeller, providing mechanistic insight into the incorporation of Rpl10 into pre-60S subunits. Co-translational capturing of nascent ribosomal proteins by dedicated chaperones constitutes an elegant mechanism to prevent unspecific interactions and aggregation of ribosomal proteins on their road to incorporation. |
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| DOI: | doi:10.1038/ncomms8494 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/ncomms8494 |
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| | Volltext: https://www.nature.com/articles/ncomms8494 |
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| | DOI: https://doi.org/10.1038/ncomms8494 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1703018753 |
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| Verknüpfungen: | → Zeitschrift |
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Co-translational capturing of nascent ribosomal proteins by their dedicated chaperones / Pausch, Patrick [VerfasserIn]; 26 Jun 2015 (Online-Ressource)
