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| Verfasst von: | Fröhlich, Benjamin [VerfasserIn]  |
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| | Yang, Yang [VerfasserIn] |
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| | Thoma, Judith [VerfasserIn] |
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| | Czajor, Julian [VerfasserIn] |
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| | Lansche, Christine [VerfasserIn] |
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| | Sanchez, Cecilia P. [VerfasserIn] |
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| | Lanzer, Michael [VerfasserIn] |
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| | Cloetens, Peter [VerfasserIn] |
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| | Tanaka, Motomu [VerfasserIn] |
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| Titel: | Nanofocused scanning X-ray fluorescence microscopy revealing an effect of heterozygous hemoglobin S and C on biochemical activities in plasmodium falciparum-infected erythrocytes |
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| Verf.angabe: | Benjamin Fröhlich, Yang Yang, Judith Thoma, Julian Czajor, Christine Lansche, Cecilia Sanchez, Michael Lanzer, Peter Cloetens, and Motomu Tanaka |
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| E-Jahr: | 2020 |
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| Jahr: | March 23, 2020 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 02.07.2020 |
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| Titel Quelle: | Enthalten in: Analytical chemistry |
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| Ort Quelle: | Columbus, Ohio : American Chemical Society, 1947 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 92(2020), 8, Seite 5765-5771 |
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| ISSN Quelle: | 1520-6882 |
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| Abstract: | While there is ample evidence suggesting that carriers of heterozygous hemoglobin S and C are protected from life- threatening malaria, little is known about the underlying biochemical mechanisms at the single cell level. Using nanofocused scanning X-ray fluorescence microscopy, we quantify the spatial distribution of individual elements in subcellular compartments, including Fe, S, P, Zn, and Cu, in Plasmodium falciparum-infected (P. falciparum-infected) erythrocytes carrying the wild type or variant hemoglobins. Our data indicate that heterozygous hemoglobin S and C significantly modulate biochemical reactions in parasitized erythrocytes, such as aberrant hemozoin mineralization and a delay in hemoglobin degradation. The label-free scanning X-ray fluorescence imaging has great potential to quantify the spatial distribution of elements in subcellular compartments of P. falciparum-infected erythrocytes and unravel the biochemical mechanisms underpinning disease and protective traits. |
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| DOI: | doi:10.1021/acs.analchem.9b05111 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1021/acs.analchem.9b05111 |
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| | DOI: https://doi.org/10.1021/acs.analchem.9b05111 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | amino-acids |
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| | digestion |
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| | efflux |
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| K10plus-PPN: | 1703276450 |
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| Verknüpfungen: | → Zeitschrift |
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Nanofocused scanning X-ray fluorescence microscopy revealing an effect of heterozygous hemoglobin S and C on biochemical activities in plasmodium falciparum-infected erythrocytes / Fröhlich, Benjamin [VerfasserIn]; March 23, 2020 (Online-Ressource)
