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Verfasst von:García, Patricia [VerfasserIn]   i
 Bizama, Carolina [VerfasserIn]   i
 Rosa, Lorena [VerfasserIn]   i
 Espinoza, Jaime A. [VerfasserIn]   i
 Weber, Helga [VerfasserIn]   i
 Cerda-Infante, Javier [VerfasserIn]   i
 Sanchez, Marianela [VerfasserIn]   i
 Montecinos, Viviana P. [VerfasserIn]   i
 Lorenzo Bermejo, Justo [VerfasserIn]   i
 Boekstegers, Felix [VerfasserIn]   i
 Davila-Lopez, Marcela [VerfasserIn]   i
 Alfaro, Francisca [VerfasserIn]   i
 Leiva-Acevedo, Claudia [VerfasserIn]   i
 Parra, Zasha [VerfasserIn]   i
 Romero, Diego [VerfasserIn]   i
 Kato, Sumie [VerfasserIn]   i
 Leal, Pamela [VerfasserIn]   i
 Lagos, Marcela [VerfasserIn]   i
 Carlos Roa, Juan [VerfasserIn]   i
Titel:Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
Verf.angabe:Patricia García, Carolina Bizama, Lorena Rosa, Jaime A. Espinoza, Helga Weber, Javier Cerda-Infante, Marianela Sanchez, Viviana P. Montecinos, Justo Lorenzo-Bermejo, Felix Boekstegers, Marcela Davila-Lopez, Francisca Alfaro, Claudia Leiva-Acevedo, Zasha Parra, Diego Romero, Sumie Kato, Pamela Leal, Marcela Lagos and Juan Carlos Roa
E-Jahr:2020
Jahr:15 April 2020
Umfang:17 S.
Fussnoten:Gesehen am 02.07.2020
Titel Quelle:Enthalten in: Biological research
Ort Quelle:Santiago : [Verlag nicht ermittelbar], 1999
Jahr Quelle:2020
Band/Heft Quelle:53(2020,1) Artikel-Nummer 13, 17 Seiten
ISSN Quelle:0717-6287
Abstract:Background Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. Results After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
DOI:doi:10.1186/s40659-020-00282-7
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1186/s40659-020-00282-7
 DOI: https://doi.org/10.1186/s40659-020-00282-7
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Ascites
 breast-cancer
 Cancer cell lines
 carcinoma
 Gallbladder cancer
 gene amplification
 Gene expression profile
 her2 intratumoral heterogeneity
 mesenchymal transition
 Native American ancestry
 p53 protein
 prognostic-significance
 prostate-cancer
 protein expression
 sialylated muc1
K10plus-PPN:1703311523
Verknüpfungen:→ Zeitschrift

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