When the label matters

• Verfasst von: Romanowska, Julia [VerfasserIn]
• Verfasst von: Kokh, Daria B. [VerfasserIn]
• Verfasst von: Wade, Rebecca C. [VerfasserIn]
• Titel: When the label matters
• Titelzusatz: adsorption of labeled and unlabeled proteins on charged surfaces
• Verf.angabe: Julia Romanowska, Daria B. Kokh, Rebecca C. Wade
• E-Jahr: 2015
• Jahr: October 22, 2015
• Umfang: 6 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 02.07.2020
• Titel Quelle: Enthalten in: Nano letters
• Ort Quelle: Washington, DC : ACS Publ., 2001
• Jahr Quelle: 2015
• Band/Heft Quelle: 15(2015), 11, Seite 7508-7513
• ISSN Quelle: 1530-6992
• DOI: doi:10.1021/acs.nanolett.5b03168
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1703317394

Abstract

Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can significantly change the interaction properties of a protein. Thus, the results of experiments with fluorescently labeled proteins should be interpreted by modeling the structures and computing the interaction properties of both labeled and unlabeled species.