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Verfasst von:Neuhaus, Winfried [VerfasserIn]   i
 Gaiser, Fabian [VerfasserIn]   i
 Mahringer, Anne [VerfasserIn]   i
 Franz, Jonas [VerfasserIn]   i
 Riethmüller, Christoph [VerfasserIn]   i
 Förster, Carola [VerfasserIn]   i
Titel:The pivotal role of astrocytes in an in vitro stroke model of the blood-brain barrier
Verf.angabe:Winfried Neuhaus, Fabian Gaiser, Anne Mahringer, Jonas Franz, Christoph Riethmüller and Carola Förster
E-Jahr:2014
Jahr:28 October 2014
Fussnoten:Gesehen am 03.07.2020
Titel Quelle:Enthalten in: Frontiers in cellular neuroscience
Ort Quelle:Lausanne : Frontiers Research Foundation, 2007
Jahr Quelle:2014
Band/Heft Quelle:8(2014) Artikel-Nummer 352, 16 Seiten
ISSN Quelle:1662-5102
Abstract:Stabilization of the blood-brain barrier during and after stroke can lead to less adverse outcome. For elucidation of underlying mechanisms and development of novel therapeutic strategies validated in-vitro disease models of the blood-brain barrier could be very helpful. To mimic in-vitro stroke conditions we have established a blood-brain barrier in-vitro model based on mouse cell line cerebEND and applied oxygen/glucose deprivation (OGD). The role of astrocytes in this disease model was investigated by using cell line C6. Transwell studies pointed out that addition of astrocytes during OGD increased the barrier damage significantly in comparison to the endothelial monoculture shown by changes of transendothelial electrical resistance as well as fluorescein permeability data. Analysis on mRNA and protein levels by qPCR, western blotting and immunofluorescence microscopy of tight junction molecules claudin-3,-5,-12, occludin and ZO-1 revealed that their regulation and localisation is associated with the functional barrier breakdown. Furthermore, soluble factors of astrocytes, OGD and their combination were able to induce changes of functionality and expression of ABC-transporters Abcb1a (P-gp), Abcg2 (bcrp) and Abcc4 (mrp4). Moreover, the expression of proteases (matrixmetalloproteinases MMP-2, MMP-3 and MMP-9 and t-PA) as well as of their endogenous inhibitors (TIMP-1, TIMP-3, PAI-1) was altered by astrocyte factors and OGD which resulted in significant changes of total MMP and t-PA activity. Morphological rearrangements induced by OGD and treatment with astrocyte factors were confirmed at a nanometer scale using atomic force microscopy. In conclusion, astrocytes play a major role in blood-brain barrier breakdown during OGD in vitro.
DOI:doi:10.3389/fncel.2014.00352
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3389/fncel.2014.00352
 Volltext: https://www.frontiersin.org/articles/10.3389/fncel.2014.00352/full
 DOI: https://doi.org/10.3389/fncel.2014.00352
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Blood-Brain Barrier
 C6
 cerebEND
 In-vitro
 Ischemia
 neurovascular unit
 oxygen/glucose deprivation
 Stroke
 Traumatic Brain Injury
K10plus-PPN:1703449053
Verknüpfungen:→ Zeitschrift

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