Spines slow down dendritic chloride diffusion and affect short-term ionic plasticity of GABAergic inhibition

• Verfasst von: Mohapatra, Namrata [VerfasserIn]
• Verfasst von: Tønnesen, Jan [VerfasserIn]
• Verfasst von: Vlachos, Andreas [VerfasserIn]
• Verfasst von: Kuner, Thomas [VerfasserIn]
• Verfasst von: Deller, Thomas [VerfasserIn]
• Verfasst von: Nägerl, U. Valentin [VerfasserIn]
• Verfasst von: Santamaria, Fidel [VerfasserIn]
• Verfasst von: Jedlicka, Peter [VerfasserIn]
• Titel: Spines slow down dendritic chloride diffusion and affect short-term ionic plasticity of GABAergic inhibition
• Verf.angabe: Namrata Mohapatra, Jan Tønnesen, Andreas Vlachos, Thomas Kuner, Thomas Deller, U. Valentin Nägerl, Fidel Santamaria & Peter Jedlicka
• E-Jahr: 2016
• Jahr: 18 March 2016
• Umfang: 14 S.
• Fussnoten: Gesehen am 10.07.2020
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Springer Nature, 2011
• Jahr Quelle: 2016
• Band/Heft Quelle: 6(2016) Artikel-Nummer 23196, 14 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/srep23196
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1724372955

Abstract

Cl− plays a crucial role in neuronal function and synaptic inhibition. However, the impact of neuronal morphology on the diffusion and redistribution of intracellular Cl− is not well understood. The role of spines in Cl− diffusion along dendritic trees has not been addressed so far. Because measuring fast and spatially restricted Cl− changes within dendrites is not yet technically possible, we used computational approaches to predict the effects of spines on Cl− dynamics in morphologically complex dendrites. In all morphologies tested, including dendrites imaged by super-resolution STED microscopy in live brain tissue, spines slowed down longitudinal Cl− diffusion along dendrites. This effect was robust and could be observed in both deterministic as well as stochastic simulations. Cl− extrusion altered Cl− diffusion to a much lesser extent than the presence of spines. The spine-dependent slowing of Cl− diffusion affected the amount and spatial spread of changes in the GABA reversal potential thereby altering homosynaptic as well as heterosynaptic short-term ionic plasticity at GABAergic synapses in dendrites. Altogether, our results suggest a fundamental role of dendritic spines in shaping Cl− diffusion, which could be of relevance in the context of pathological conditions where spine densities and neural excitability are perturbed.