Novel respiratory syncytial virus (RSV) genotype ON1 predominates in Germany during winter season 2012-13

• Verfasst von: Tabatabai, Julia [VerfasserIn]
• Verfasst von: Prifert, Christiane [VerfasserIn]
• Verfasst von: Pfeil, Johannes [VerfasserIn]
• Verfasst von: Grulich-Henn, Jürgen [VerfasserIn]
• Verfasst von: Schnitzler, Paul [VerfasserIn]
• Titel: Novel respiratory syncytial virus (RSV) genotype ON1 predominates in Germany during winter season 2012-13
• Verf.angabe: Julia Tabatabai, Christiane Prifert, Johannes Pfeil, Jürgen Grulich-Henn, Paul Schnitzler
• E-Jahr: 2014
• Jahr: October 7, 2014
• Fussnoten: Gesehen am 14.07.2020
• Titel Quelle: Enthalten in: PLOS ONE
• Ort Quelle: San Francisco, California, US : PLOS, 2006
• Jahr Quelle: 2014
• Band/Heft Quelle: 9(2014,10) Artikel-Nummer e109191, 9 Seiten
• ISSN Quelle: 1932-6203
• DOI: doi:10.1371/journal.pone.0109191
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Germany
• Sach-SW: Lower respiratory tract infections
• Sach-SW: Pediatric infections
• Sach-SW: Phylogenetic analysis
• Sach-SW: Polymerase chain reaction
• Sach-SW: Protein sequencing
• Sach-SW: Respiratory syncytial virus infection
• Sach-SW: Sequence alignment
• K10plus-PPN: 1724642693

Abstract

Respiratory syncytial virus (RSV) is the leading cause of hospitalization especially in young children with respiratory tract infections (RTI). Patterns of circulating RSV genotypes can provide a better understanding of the molecular epidemiology of RSV infection. We retrospectively analyzed the genetic diversity of RSV infection in hospitalized children with acute RTI admitted to University Hospital Heidelberg/Germany between October 2012 and April 2013. Nasopharyngeal aspirates (NPA) were routinely obtained in 240 children younger than 2 years of age who presented with clinical symptoms of upper or lower RTI. We analyzed NPAs via PCR and sequence analysis of the second variable region of the RSV G gene coding for the attachment glycoprotein. We obtained medical records reviewing routine clinical data. RSV was detected in 134/240 children. In RSV-positive patients the most common diagnosis was bronchitis/bronchiolitis (75.4%). The mean duration of hospitalization was longer in RSV-positive compared to RSV-negative patients (3.5 vs. 5.1 days; p<0.01). RSV-A was detected in 82.1%, RSV-B in 17.9% of all samples. Phylogenetic analysis of 112 isolates revealed that the majority of RSV-A strains (65%) belonged to the novel ON1 genotype containing a 72-nucleotide duplication. However, genotype ON1 was not associated with a more severe course of illness when taking basic clinical/laboratory parameters into account. Molecular characterization of RSV confirms the co-circulation of multiple genotypes of subtype RSV-A and RSV-B. The duplication in the G gene of genotype ON1 might have an effect on the rapid spread of this emerging RSV strain.