Drug target identification using a trypanosome overexpression library

• Verfasst von: Begolo, Daniela [VerfasserIn]
• Verfasst von: Erben, Esteban D. [VerfasserIn]
• Verfasst von: Clayton, Christine [VerfasserIn]
• Titel: Drug target identification using a trypanosome overexpression library
• Verf.angabe: Daniela Begolo, Esteban Erben, Christine Clayton
• E-Jahr: 2014
• Jahr: 21 July 2014
• Umfang: 5 S.
• Fussnoten: Gesehen am 15.07.2020
• Titel Quelle: Enthalten in: Antimicrobial agents and chemotherapy
• Ort Quelle: Washington, DC : Soc., 1972
• Jahr Quelle: 2014
• Band/Heft Quelle: 58(2014), 10, Seite 6260-6264
• ISSN Quelle: 1098-6596
• DOI: doi:10.1128/AAC.03338-14
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1724834479

Abstract

Elucidation of molecular targets is very important for lead optimization during the drug development process. We describe a direct method to find targets of antitrypanosomal compounds against Trypanosoma brucei using a trypanosome overexpression library. As proof of concept, we treated the library with difluoromethylornithine and DDD85646 and identified their respective targets, ornithine decarboxylase and N-myristoyltransferase. The overexpression library could be a useful tool to study the modes of action of novel antitrypanosomal drug candidates.