| |  Online-Ressource |
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| Verfasst von: | Zacherl, Sonja [VerfasserIn]  |
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| | La Venuta, Giuseppe [VerfasserIn] |
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| | Müller, Hans-Michael [VerfasserIn] |
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| | Wegehingel, Sabine [VerfasserIn] |
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| | Dimou, Eleni [VerfasserIn] |
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| | Sehr, Peter [VerfasserIn] |
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| | Lewis, Joe D. [VerfasserIn] |
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| | Erfle, Holger [VerfasserIn] |
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| | Pepperkok, Rainer [VerfasserIn] |
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| | Nickel, Walter [VerfasserIn] |
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| Titel: | A direct role for ATP1A1 in unconventional secretion of fibroblast growth factor 2 |
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| Verf.angabe: | Sonja Zacherl, Giuseppe La Venuta, Hans-Michael Müller, Sabine Wegehingel, Eleni Dimou, Peter Sehr, Joe D. Lewis, Holger Erfle, Rainer Pepperkok, and Walter Nickel |
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| Jahr: | 2015 |
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| Jahr des Originals: | 2014 |
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| Umfang: | 12 S. |
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| Fussnoten: | Gesehen am 16.07.2020 ; published online December 22, 2014 |
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| Titel Quelle: | Enthalten in: The journal of biological chemistry |
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| Ort Quelle: | Bethesda, Md. : Soc., 1905 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 290(2015), 6, Seite 3654-3665 |
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| ISSN Quelle: | 1083-351X |
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| Abstract: | Previous studies proposed a role for the Na/K-ATPase in unconventional secretion of fibroblast growth factor 2 (FGF2). This conclusion was based upon pharmacological inhibition of FGF2 secretion in the presence of ouabain. However, neither independent experimental evidence nor a potential mechanism was provided. Based upon an unbiased RNAi screen, we now report the identification of ATP1A1, the α1-chain of the Na/K-ATPase, as a factor required for efficient secretion of FGF2. As opposed to ATP1A1, down-regulation of the β1- and β3-chains (ATP1B1 and ATP1B3) of the Na/K-ATPase did not affect FGF2 secretion, suggesting that they are dispensable for this process. These findings indicate that it is not the membrane potential-generating function of the Na/K-ATPase complex but rather a so far unidentified role of potentially unassembled α1-chains that is critical for unconventional secretion of FGF2. Consistently, in the absence of β-chains, we found a direct interaction between the cytoplasmic domain of ATP1A1 and FGF2 with submicromolar affinity. Based upon these observations, we propose that ATP1A1 is a recruitment factor for FGF2 at the inner leaflet of plasma membranes that may control phosphatidylinositol 4,5-bisphosphate-dependent membrane translocation as part of the unconventional secretory pathway of FGF2. |
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| DOI: | doi:10.1074/jbc.M114.590067 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1074/jbc.M114.590067 |
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| | Volltext: http://www.jbc.org/content/290/6/3654 |
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| | DOI: https://doi.org/10.1074/jbc.M114.590067 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | ATP1A1 |
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| | Fibroblast Growth Factor (FGF) |
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| | Heparan Sulfate |
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| | Membrane Recruitment |
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| | Membrane Translocation |
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| | Na+/K+-ATPase |
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| | Phosphoinositide |
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| | Plasma Membrane |
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| | Tec Kinase |
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| | Unconventional Secretion |
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| K10plus-PPN: | 1724957279 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ direct role for ATP1A1 in unconventional secretion of fibroblast growth factor 2 / Zacherl, Sonja [VerfasserIn]; 2015 (Online-Ressource)
