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| Verfasst von: | Erbel, Christian [VerfasserIn]  |
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| | Tyka, Mirjam Evelin [VerfasserIn] |
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| | Helmes, Christian Michael [VerfasserIn] |
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| | Akhavanpoor, Mohammadreza [VerfasserIn] |
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| | Rupp, Gregor Fabian [VerfasserIn] |
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| | Domschke, Gabriele [VerfasserIn] |
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| | Linden, Fabian [VerfasserIn] |
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| | Wolf, Antonia [VerfasserIn] |
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| | Dösch, Andreas [VerfasserIn] |
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| | Lasitschka, Felix [VerfasserIn] |
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| | Katus, Hugo [VerfasserIn] |
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| | Gleißner, Christian A. [VerfasserIn] |
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| Titel: | CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+ in vitro and in vivo |
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| Titelzusatz: | $hChristian Erbel, Mirjam Tyka, Christian M. Helmes, Mohmmadreza Akhavanpoor, Gregor Rupp, Gabriele Domschke, Fabian Linden, Antonia Wolf, Andreas Doesch, Felix Lasitschka, Hugo A. Katus and Christian A. Gleissner |
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| Jahr: | 2015 |
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| Jahr des Originals: | 2014 |
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| Umfang: | 11 S. |
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| Fussnoten: | Im Titel sind die "+"-Zeichen hochgestellt ; Article first published online: March 24, 2014 ; Gesehen am 16.07.2020 |
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| Titel Quelle: | Enthalten in: Innate immunity |
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| Ort Quelle: | Thousand Oaks, Calif. : Sage, 2008 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 21(2015), 3, Seite 255-265 |
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| ISSN Quelle: | 1753-4267 |
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| Abstract: | Macrophage heterogeneity in human atherosclerotic plaques has been recognized; however, markers for unequivocal identification of some subtypes are lacking. We found that the platelet chemokine CXCL4 induces a unique macrophage phenotype, which we proposed to call ‘M4’. Here, we sought to identify suitable markers that identify M4 macrophages in vitro and in vivo. Using a stringent algorithm, we identified a set of potential markers from transcriptomic data derived from polarized macrophages. We specifically focused on matrix metalloproteinase (MMP)7 and S100A8, the co-expression of which has not been described in any macrophage type thus far. We found dose- and time-dependent MMP7 and S100A8 expression in M4 macrophages at the gene and protein levels. CXCL4-induced up-regulation of both MMP7 and S100A8 was curbed in the presence of heparin, which binds to CXCL4 and glycosaminoglycans, most likely representing the macrophage receptor for CXCL4. Immunofluorescence of post-mortem atherosclerotic coronary arteries identified CD68+MMP7+, CD68+MMP7−, CD68+S100A8+ and CD68+S100A8− macrophages. A small proportion of MMP7+S100A8+ macrophages most likely represent M4 macrophages. In summary, we have identified co-expression of MMP7 and S100A8 to be a marker combination exclusively found in M4 macrophages. This finding may allow further dissection of the role of M4 macrophages in atherosclerosis and other pathologic conditions. |
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| DOI: | doi:10.1177/1753425914526461 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1177/1753425914526461 |
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| | Volltext: https://journals.sagepub.com/doi/10.1177/1753425914526461 |
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| | DOI: https://doi.org/10.1177/1753425914526461 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1724958720 |
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| Verknüpfungen: | → Zeitschrift |
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CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+ in vitro and in vivo / Erbel, Christian [VerfasserIn]; 2015 (Online-Ressource)
