Status: Bibliographieeintrag
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| Verfasst von: | Gigova, Andriana [VerfasserIn]  |
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| | Duggimpudi, Sujitha Smruthy [VerfasserIn] |
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| | Pollex, Tim [VerfasserIn] |
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| | Schaefer, Matthias R. [VerfasserIn] |
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| | Koš, Martin [VerfasserIn] |
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| Titel: | A cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability |
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| Verf.angabe: | Andriana Gigova, Sujitha Duggimpudi, Tim Pollex, Matthias Schaefer, and Martin Koš |
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| E-Jahr: | 2014 |
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| Jahr: | August 14, 2014 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 22.07.2020 |
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| Titel Quelle: | Enthalten in: RNA |
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| Ort Quelle: | Cold Spring Harbor, NY : Laboratory Press, 1995 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 20(2014), 10, Seite 1632-1644 |
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| ISSN Quelle: | 1469-9001 |
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| Abstract: | In all three domains of life ribosomal RNAs are extensively modified at functionally important sites of the ribosome. These modifications are believed to fine-tune the ribosome structure for optimal translation. However, the precise mechanistic effect of modifications on ribosome function remains largely unknown. Here we show that a cluster of methylated nucleotides in domain IV of 25S rRNA is critical for integrity of the large ribosomal subunit. We identified the elusive cytosine-5 methyltransferase for C2278 in yeast as Rcm1 and found that a combined loss of cytosine-5 methylation at C2278 and ribose methylation at G2288 caused dramatic ribosome instability, resulting in loss of 60S ribosomal subunits. Structural and biochemical analyses revealed that this instability was caused by changes in the structure of 25S rRNA and a consequent loss of multiple ribosomal proteins from the large ribosomal subunit. Our data demonstrate that individual RNA modifications can strongly affect structure of large ribonucleoprotein complexes. |
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| DOI: | doi:10.1261/rna.043398.113 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1261/rna.043398.113 |
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| | Volltext: http://rnajournal.cshlp.org/content/20/10/1632 |
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| | DOI: https://doi.org/10.1261/rna.043398.113 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | ribosomal RNA |
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| | ribosome stability |
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| | RNA modification |
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| | translation |
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| | yeast |
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| K10plus-PPN: | 1725279916 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ cluster of methylations in the domain IV of 25S rRNA is required for ribosome stability / Gigova, Andriana [VerfasserIn]; August 14, 2014 (Online-Ressource)
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