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Verfasst von:Gorniak, Thomas [VerfasserIn]   i
 Haraszti, Tamás [VerfasserIn]   i
 Garamus, Vasyl M. [VerfasserIn]   i
 Gundlach, Andreas Robert von [VerfasserIn]   i
 Senkbeil, Tobias [VerfasserIn]   i
 Priebe, Marius [VerfasserIn]   i
 Hedberg-Buenz, Adam [VerfasserIn]   i
 Koehn, Demelza [VerfasserIn]   i
 Salditt, Tim [VerfasserIn]   i
 Grunze, Michael [VerfasserIn]   i
 Anderson, Michael G. [VerfasserIn]   i
 Rosenhahn, Axel [VerfasserIn]   i
Titel:Nano-scale morphology of melanosomes revealed by small-angle X-ray scattering
Verf.angabe:Thomas Gorniak, Tamas Haraszti, Vasyl M. Garamus, Andreas R. Buck, Tobias Senkbeil, Marius Priebe, Adam Hedberg-Buenz, Demelza Koehn, Tim Salditt, Michael Grunze, Michael G. Anderson, Axel Rosenhahn
E-Jahr:2014
Jahr:March 12, 2014
Umfang:8 S.
Fussnoten:Gesehen am 05.08.2020
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2014
Band/Heft Quelle:9(2014,3) Artikel-Nummer e90884, 8 Seiten
ISSN Quelle:1932-6203
Abstract:Melanosomes are highly specialized organelles that produce and store the pigment melanin, thereby fulfilling essential functions within their host organism. Besides having obvious cosmetic consequences - determining the color of skin, hair and the iris - they contribute to photochemical protection from ultraviolet radiation, as well as to vision (by defining how much light enters the eye). Though melanosomes can be beneficial for health, abnormalities in their structure can lead to adverse effects. Knowledge of their ultrastructure will be crucial to gaining insight into the mechanisms that ultimately lead to melanosome-related diseases. However, due to their small size and electron-dense content, physiologically intact melanosomes are recalcitrant to study by common imaging techniques such as light and transmission electron microscopy. In contrast, X-ray-based methodologies offer both high spatial resolution and powerful penetrating capabilities, and thus are well suited to study the ultrastructure of electron-dense organelles in their natural, hydrated form. Here, we report on the application of small-angle X-ray scattering - a method effective in determining the three-dimensional structures of biomolecules - to whole, hydrated murine melanosomes. The use of complementary information from the scattering signal of a large ensemble of suspended organelles and from single, vitrified specimens revealed a melanosomal sub-structure whose surface and bulk properties differ in two commonly used inbred strains of laboratory mice. Whereas melanosomes in C57BL/6J mice have a well-defined surface and are densely packed with 40-nm units, their counterparts in DBA/2J mice feature a rough surface, are more granular and consist of 60-nm building blocks. The fact that these strains have different coat colors and distinct susceptibilities to pigment-related eye disease suggest that these differences in size and packing are of biological significance.
DOI:doi:10.1371/journal.pone.0090884
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0090884
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090884
 DOI: https://doi.org/10.1371/journal.pone.0090884
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cellular structures and organelles
 Melanin
 Photons
 Pigments
 Scanning electron microscopy
 Small-angle scattering
 Transmission electron microscopy
 X-ray radiography
K10plus-PPN:1726238016
Verknüpfungen:→ Zeitschrift

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