Status: Bibliographieeintrag
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| Verfasst von: | Hoffmann, Julia [VerfasserIn]  |
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| | Shostak, Anton [VerfasserIn] |
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| | Fischer, Tamás [VerfasserIn] |
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| | Brunner, Michael [VerfasserIn] |
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| Titel: | Non-circadian expression masking clock-driven weak transcription rhythms in U2OS cells |
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| Verf.angabe: | Julia Hoffmann, Laura Symul, Anton Shostak, Tamás Fischer, Felix Naef, Michael Brunner |
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| E-Jahr: | 2014 |
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| Jahr: | July 9, 2014 |
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| Umfang: | 12 S. |
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| Fussnoten: | Gesehen am 06.08.2020 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 9(2014,7) Artikel-Nummer e102238, 12 Seiten |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | U2OS cells harbor a circadian clock but express only a few rhythmic genes in constant conditions. We identified 3040 binding sites of the circadian regulators BMAL1, CLOCK and CRY1 in the U2OS genome. Most binding sites even in promoters do not correlate with detectable rhythmic transcript levels. Luciferase fusions reveal that the circadian clock supports robust but low amplitude transcription rhythms of representative promoters. However, rhythmic transcription of these potentially clock-controlled genes is masked by non-circadian transcription that overwrites the weaker contribution of the clock in constant conditions. Our data suggest that U2OS cells harbor an intrinsically rather weak circadian oscillator. The oscillator has the potential to regulate a large number of genes. The contribution of circadian versus non-circadian transcription is dependent on the metabolic state of the cell and may determine the apparent complexity of the circadian transcriptome. |
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| DOI: | doi:10.1371/journal.pone.0102238 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0102238 |
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| | Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102238 |
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| | DOI: https://doi.org/10.1371/journal.pone.0102238 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Chronobiology |
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| | Circadian oscillators |
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| | Circadian rhythms |
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| | DNA transcription |
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| | Gene expression |
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| | Genetic oscillators |
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| | Regulator genes |
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| | Transcriptional control |
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| K10plus-PPN: | 1726562174 |
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| Verknüpfungen: | → Zeitschrift |
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Non-circadian expression masking clock-driven weak transcription rhythms in U2OS cells / Hoffmann, Julia [VerfasserIn]; July 9, 2014 (Online-Ressource)
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