| |  Online-Ressource |
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| Verfasst von: | Legrand, Cyril [VerfasserIn]  |
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| | Saleppico, Roberto [VerfasserIn] |
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| | Sticht, Jana [VerfasserIn] |
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| | Lolicato, Fabio [VerfasserIn] |
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| | Müller, Hans-Michael [VerfasserIn] |
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| | Wegehingel, Sabine [VerfasserIn] |
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| | Dimou, Eleni [VerfasserIn] |
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| | Steringer, Julia P. [VerfasserIn] |
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| | Ewers, Helge [VerfasserIn] |
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| | Vattulainen, Ilpo [VerfasserIn] |
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| | Freund, Christian [VerfasserIn] |
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| | Nickel, Walter [VerfasserIn] |
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| Titel: | The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2 |
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| Verf.angabe: | Cyril Legrand, Roberto Saleppico, Jana Sticht, Fabio Lolicato, Hans-Michael Müller, Sabine Wegehingel, Eleni Dimou, Julia P. Steringer, Helge Ewers, Ilpo Vattulainen, Christian Freund & Walter Nickel |
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| E-Jahr: | 2020 |
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| Jahr: | 25 March 2020 |
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| Umfang: | 16 S. |
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| Fussnoten: | Im Titel ist die Ziffer 2 bei "PI(4,5)P2" tiefgestellt ; Gesehen am 10.08.2020 |
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| Titel Quelle: | Enthalten in: Communications biology |
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| Ort Quelle: | London : Springer Nature, 2018 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 3(2020) Artikel-Nummer 141, 16 Seiten |
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| ISSN Quelle: | 2399-3642 |
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| Abstract: | FGF2 is a tumor cell survival factor that is exported from cells by an ER/Golgi-independent secretory pathway. This unconventional mechanism of protein secretion is based on direct translocation of FGF2 across the plasma membrane. The Na,K-ATPase has previously been shown to play a role in this process, however, the underlying mechanism has remained elusive. Here, we define structural elements that are critical for a direct physical interaction between FGF2 and the α1 subunit of the Na,K-ATPase. In intact cells, corresponding FGF2 mutant forms were impaired regarding both recruitment at the inner plasma membrane leaflet and secretion. Ouabain, a drug that inhibits both the Na,K-ATPase and FGF2 secretion, was found to impair the interaction of FGF2 with the Na,K-ATPase in cells. Our findings reveal the Na,K-ATPase as the initial recruitment factor for FGF2 at the inner plasma membrane leaflet being required for efficient membrane translocation of FGF2 to cell surfaces. |
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| DOI: | doi:10.1038/s42003-020-0871-y |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/s42003-020-0871-y |
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| | Volltext: https://www.nature.com/articles/s42003-020-0871-y |
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| | DOI: https://doi.org/10.1038/s42003-020-0871-y |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1726681351 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2 / Legrand, Cyril [VerfasserIn]; 25 March 2020 (Online-Ressource)
