| |  Online-Ressource |
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| Verfasst von: | Bunina, Daria [VerfasserIn]  |
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| | Abazova, Nadeshda [VerfasserIn] |
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| | Diaz, Nichole [VerfasserIn] |
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| | Noh, Kyung-Min [VerfasserIn] |
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| | Krijgsveld, Jeroen [VerfasserIn] |
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| | Zaugg, Judith B. [VerfasserIn] |
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| Titel: | Genomic rewiring of SOX2 chromatin interaction network during differentiation of ESCs to postmitotic neurons |
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| Verf.angabe: | Daria Bunina, Nade Abazova, Nichole Diaz, Kyung-Min Noh, Jeroen Krijgsveld, and Judith B. Zaugg |
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| E-Jahr: | 2020 |
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| Jahr: | June 17, 2020 |
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| Umfang: | 23 S. |
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| Fussnoten: | Gesehen am 10.08.2020 |
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| Titel Quelle: | Enthalten in: Cell systems |
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| Ort Quelle: | Maryland Heights, MO : Elsevier, 2015 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 10(2020), 6, Seite 480-494, e1-e8 |
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| ISSN Quelle: | 2405-4720 |
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| Abstract: | Cellular differentiation requires dramatic changes in chromatin organization, transcriptional regulation, and protein production. To understand the regulatory connections between these processes, we generated proteomic, transcriptomic, and chromatin accessibility data during differentiation of mouse embryonic stem cells (ESCs) into postmitotic neurons and found extensive associations between different molecular layers within and across differentiation time points. We observed that SOX2, as a regulator of pluripotency and neuronal genes, redistributes from pluripotency enhancers to neuronal promoters during differentiation, likely driven by changes in its protein interaction network. We identified ATRX as a major SOX2 partner in neurons, whose co-localization correlated with an increase in active enhancer marks and increased expression of nearby genes, which we experimentally confirmed for three loci. Collectively, our data provide key insights into the regulatory transformation of SOX2 during neuronal differentiation, and we highlight the significance of multi-omic approaches in understanding gene regulation in complex systems. |
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| DOI: | doi:10.1016/j.cels.2020.05.003 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.cels.2020.05.003 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S2405471220301885 |
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| | DOI: https://doi.org/10.1016/j.cels.2020.05.003 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Atrx |
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| | data integration |
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| | gene regulation |
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| | multi-omics |
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| | neuronal differentiation |
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| | Sox2 |
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| | transcription factors |
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| K10plus-PPN: | 1726704262 |
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| Verknüpfungen: | → Zeitschrift |
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Genomic rewiring of SOX2 chromatin interaction network during differentiation of ESCs to postmitotic neurons / Bunina, Daria [VerfasserIn]; June 17, 2020 (Online-Ressource)
