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| Verfasst von: | Leidig, Christoph [VerfasserIn]  |
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| | Thoms, Matthias [VerfasserIn] |
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| | Holdermann, Iris Valerie [VerfasserIn] |
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| | Bradatsch, Bettina [VerfasserIn] |
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| | Berninghausen, Otto [VerfasserIn] |
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| | Bange, Gert [VerfasserIn] |
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| | Sinning, Irmgard [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| | Beckmann, Roland [VerfasserIn] |
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| Titel: | 60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle |
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| Verf.angabe: | Christoph Leidig, Matthias Thoms, Iris Holdermann, Bettina Bradatsch, Otto Berninghausen, Gert Bange, Irmgard Sinning, Ed Hurt, & Roland Beckmann |
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| E-Jahr: | 2014 |
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| Jahr: | 24 March 2014 |
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| Umfang: | 8 S. |
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| Fussnoten: | Gesehen am 13.08.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 5(2014), Artikel-ID 3491, Seite 1-8 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | During eukaryotic ribosome biogenesis, nascent ribosomal RNA (rRNA) forms pre-ribosomal particles containing ribosomal proteins and assembly factors. Subsequently, these immature rRNAs are processed and remodelled. Little is known about the premature assembly states of rRNAs and their structural rearrangement during ribosome biogenesis. Using cryo-EM we characterize a pre-60S particle, where the 5S rRNA and its associated ribosomal proteins L18 and L5 (5S ribonucleoprotein (RNP)) are rotated by almost 180° when compared with the mature subunit. Consequently, neighbouring 25S rRNA helices that protrude from the base of the central protuberance are deformed. This altered topology is stabilized by nearby assembly factors (Rsa4 and Nog1), which were identified by fitting their three-dimensional structures into the cryo-EM density. We suggest that the 5S RNP performs a semicircular movement during 60S biogenesis to adopt its final position, fulfilling a chaperone-like function in guiding the flanking 25S rRNA helices of the central protuberance to their final topology. |
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| DOI: | doi:10.1038/ncomms4491 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/ncomms4491 |
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| | Volltext: https://www.nature.com/articles/ncomms4491 |
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| | DOI: https://doi.org/10.1038/ncomms4491 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1726987078 |
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| Verknüpfungen: | → Zeitschrift |
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60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle / Leidig, Christoph [VerfasserIn]; 24 March 2014 (Online-Ressource)
