Osteogenic and chondrogenic potential of the supramolecular aggregate T-LysYal®

• Verfasst von: Di Benedetto, Adriana [VerfasserIn]
• Verfasst von: Cavalcanti-Adam, Elisabetta A. [VerfasserIn]
• Titel: Osteogenic and chondrogenic potential of the supramolecular aggregate T-LysYal®
• Verf.angabe: Adriana Di Benedetto, Francesca Posa, Mario Marazzi, Zamira Kalemaj, Roberta Grassi, Lorenzo Lo Muzio, Mariasevera Di Comite, Elisabetta Ada Cavalcanti-Adam, Felice Roberto Grassi and Giorgio Mori
• E-Jahr: 2020
• Jahr: 05 May 2020
• Umfang: 11 S.
• Fussnoten: Gesehen am 13.08.2020
• Titel Quelle: Enthalten in: Frontiers in endocrinology
• Ort Quelle: Lausanne : Frontiers Research Foundation, 2010
• Jahr Quelle: 2020
• Band/Heft Quelle: 11(2020) Artikel-Nummer 285, 11 Seiten
• ISSN Quelle: 1664-2392
• DOI: doi:10.3389/fendo.2020.00285
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Bone
• Sach-SW: Cartilage
• Sach-SW: Focal Adhesions
• Sach-SW: Mesenchymal Stem Cells
• Sach-SW: supramolecolar aggregate
• Sach-SW: Tissue Regeneration
• K10plus-PPN: 1727030869

Abstract

Hard tissue regeneration represents a challenge for the Regenerative Medicine and Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. T-LysYal® (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior stability, has already been proved efficient in repairing corneal epithelial cells damaged by dry conditions in vitro. We investigated the regenerative potential of T-Lys in the hard tissues bone and cartilage. We have previously demonstrated that cells isolated from the tooth germ, Dental Bud Stem Cells (DBSCs), differentiate into osteoblast-like cells, representing a promising source of MSCs for bone regeneration. Herewith, we show that T-Lys treatment stimulates the expression of typical osteoblastic markers, such as Alkaline Phosphatase (ALP) and Osteopontin (OPN), determining a higher production of mineralized matrix nodules. In addition, we found that T-Lys treatment positively affects αVβ3 integrin expression, key integrin in the osteoblastic commitment, leading to the formation of focal adhesions (FAs). The efficacy of T-Lys was also tested on chondrogenic differentiation starting from human articular chondrocytes (HACs) resulting in an increase of differentiation markers and cell number.