Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Erben, Esteban D. [VerfasserIn]   i
 Fadda, Abeer [VerfasserIn]   i
 Lueong, Smiths [VerfasserIn]   i
 Hoheisel, Jörg D. [VerfasserIn]   i
 Clayton, Christine [VerfasserIn]   i
Titel:A genome-wide tethering screen reveals novel potential post-transcriptional regulators in Trypanosoma brucei
Verf.angabe:Esteban D. Erben, Abeer Fadda, Smiths Lueong, Jörg D. Hoheisel, Christine Clayton
E-Jahr:2014
Jahr:June 12, 2014
Teil:volume:10
 year:2014
 number:6
Fussnoten:Gesehen am 14.08.2020
Titel Quelle:Enthalten in: Public Library of SciencePLoS pathogens
Ort Quelle:Lawrence, Kan. : PLoS, 2005
Jahr Quelle:2014
Band/Heft Quelle:10(2014,6) Artikel-Nummer e1004178, 15 Seiten
ISSN Quelle:1553-7374
Abstract:In trypanosomatids, gene expression is regulated mainly by post-transcriptional mechanisms, which affect mRNA processing, translation and degradation. Currently, our understanding of factors that regulate either mRNA stability or translation is rather limited. We know that often, the regulators are proteins that bind to the 3′-untranslated region; they presumably interact with ribonucleases and translation factors. However, very few such proteins have been characterized in any detail. Here we describe a genome-wide screen to find proteins implicated in post-transcriptional regulation in Trypanosoma brucei. We made a library of random genomic fragments in a plasmid that was designed for expression of proteins fused to an RNA-binding domain, the lambda-N peptide. This was transfected into cells expressing mRNAs encoding a positive or negative selectable marker, and bearing the “boxB” lambda-N recognition element in the 3′-untranslated region. The screen identified about 300 proteins that could be implicated in post-transcriptional mRNA regulation. These included known regulators, degradative enzymes and translation factors, many canonical RNA-binding proteins, and proteins that act via multi-protein complexes. However there were also nearly 150 potential regulators with no previously annotated function, or functions unrelated to mRNA metabolism. Almost 50 novel regulators were shown to bind RNA using a targeted proteome array. The screen also provided fine structure mapping of the hit candidates' functional domains. Our findings not only confirm the key role that RNA-binding proteins play in the regulation of gene expression in trypanosomatids, but also suggest new roles for previously uncharacterized proteins.
DOI:doi:10.1371/journal.ppat.1004178
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.ppat.1004178
 Volltext: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1004178
 DOI: https://doi.org/10.1371/journal.ppat.1004178
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Gene expression
 Messenger RNA
 Protein domains
 Protein translation
 RNA-binding proteins
 Tetracyclines
 Transfection
 Trypanosoma
K10plus-PPN:1727087356
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68627566   QR-Code
zum Seitenanfang