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| Verfasst von: | Weymann, Alexander [VerfasserIn]  |
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| | Korkmaz-İçöz, Sevil [VerfasserIn] |
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| | Li, Shiliang [VerfasserIn] |
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| | Veres, Gábor [VerfasserIn] |
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| | Chaimow, Nicole [VerfasserIn] |
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| | Pätzold, Ines [VerfasserIn] |
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| | Czerny, Natalie [VerfasserIn] |
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| | Schies, Carsten [VerfasserIn] |
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| | Schmack, Bastian [VerfasserIn] |
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| | Karck, Matthias [VerfasserIn] |
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| | Szabó, Gábor [VerfasserIn] |
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| Titel: | Bioartificial heart |
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| Titelzusatz: | a human-sized porcine model : the way ahead |
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| Verf.angabe: | Alexander Weymann, Nikhil Prakash Patil, Anton Sabashnikov, Philipp Jungebluth, Sevil Korkmaz, Shiliang Li, Gabor Veres, Pal Soos, Roland Ishtok, Nicole Chaimow, Ines Pätzold, Natalie Czerny, Carsten Schies, Bastian Schmack, Aron-Frederik Popov, André Rüdiger Simon, Matthias Karck, Gabor Szabo |
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| E-Jahr: | 2014 |
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| Jahr: | November 3, 2014 |
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| Fussnoten: | Gesehen am 18.08.2020 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 9(2014,11) Artikel-Nummer 111591, 8 Seiten |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | Background: A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Native hearts decellularized with preserved architecture and vasculature may provide an acellular tissue platform for organ regeneration. We sought to develop a tissue-engineered whole-heart neoscaffold in human-sized porcine hearts. - Methods: We decellularized porcine hearts (n = 10) by coronary perfusion with ionic detergents in a modified Langendorff circuit. We confirmed decellularization by histology, transmission electron microscopy and fluorescence microscopy, quantified residual DNA by spectrophotometry, and evaluated biomechanical stability with ex-vivo left-ventricular pressure/ volume studies, all compared to controls. We then mounted the decellularized porcine hearts in a bioreactor and reseeded them with murine neonatal cardiac cells and human umbilical cord derived endothelial cells (HUVEC) under simulated physiological conditions. - Results: Decellularized hearts lacked intracellular components but retained specific collagen fibers, proteoglycan, elastin and mechanical integrity; quantitative DNA analysis demonstrated a significant reduction of DNA compared to controls (82.663.2 ng DNA/mg tissue vs. 473.2613.4 ng DNA/mg tissue, p,0.05). Recellularized porcine whole-heart neoscaffolds demonstrated re-endothelialization of coronary vasculature and measurable intrinsic myocardial electrical activity at 10 days, with perfused organ culture maintained for up to 3 weeks. - Conclusions: Human-sized decellularized porcine hearts provide a promising tissue-engineering platform that may lead to future clinical strategies in the treatment of heart failure. |
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| DOI: | doi:10.1371/journal.pone.0111591 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0111591 |
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| | Volltext: https://dx.plos.org/10.1371/journal.pone.0111591 |
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| | DOI: https://doi.org/10.1371/journal.pone.0111591 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1727318498 |
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| Verknüpfungen: | → Zeitschrift |
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Bioartificial heart / Weymann, Alexander [VerfasserIn]; November 3, 2014 (Online-Ressource)
