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| Verfasst von: | Ollech, Dirk [VerfasserIn]  |
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| | Pflästerer, Tim [VerfasserIn] |
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| | Shellard, Adam [VerfasserIn] |
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| | Zambarda, Chiara [VerfasserIn] |
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| | Spatz, Joachim P. [VerfasserIn] |
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| | Marcq, Philippe [VerfasserIn] |
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| | Mayor, Roberto [VerfasserIn] |
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| | Wombacher, Richard [VerfasserIn] |
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| | Cavalcanti-Adam, Elisabetta A. [VerfasserIn] |
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| Titel: | An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells |
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| Verf.angabe: | Dirk Ollech, Tim Pflästerer, Adam Shellard, Chiara Zambarda, Joachim Pius Spatz, Philippe Marcq, Roberto Mayor, Richard Wombacher & Elisabetta Ada Cavalcanti-Adam |
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| E-Jahr: | 2020 |
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| Jahr: | January 24, 2020 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 18.08.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 11(2020) Artikel-Nummer 472, 13 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | The cadherin-catenin complex at adherens junctions (AJs) is essential for the formation of cell-cell adhesion and epithelium integrity; however, studying the dynamic regulation of AJs at high spatio-temporal resolution remains challenging. Here we present an optochemical tool which allows reconstitution of AJs by chemical dimerization of the force bearing structures and their precise light-induced dissociation. For the dimerization, we reconstitute acto-myosin connection of a tailless E-cadherin by two ways: direct recruitment of α-catenin, and linking its cytosolic tail to the transmembrane domain. Our approach enables a specific ON-OFF switch for mechanical coupling between cells that can be controlled spatially on subcellular or tissue scale via photocleavage. The combination with cell migration analysis and traction force microscopy shows a wide-range of applicability and confirms the mechanical contribution of the reconstituted AJs. Remarkably, in vivo our tool is able to control structural and functional integrity of the epidermal layer in developing Xenopus embryos. |
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| DOI: | doi:10.1038/s41467-020-14390-1 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-020-14390-1 |
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| | Volltext: https://www.nature.com/articles/s41467-020-14390-1 |
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| | DOI: https://doi.org/10.1038/s41467-020-14390-1 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 172732935X |
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| Verknüpfungen: | → Zeitschrift |
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¬An¬ optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells / Ollech, Dirk [VerfasserIn]; January 24, 2020 (Online-Ressource)
