| |  Online-Ressource |
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| Verfasst von: | Scuto, Maria [VerfasserIn]  |
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| | Trovato, Angela [VerfasserIn] |
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| | Modafferi, Sergio [VerfasserIn] |
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| | Polimeni, Alessandra [VerfasserIn] |
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| | Pfeffer, Tilman [VerfasserIn] |
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| | Weigand, Tim [VerfasserIn] |
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| | Calabrese, Vittorio [VerfasserIn] |
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| | Schmitt, Claus P. [VerfasserIn] |
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| | Peters, Verena [VerfasserIn] |
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| Titel: | Carnosine activates cellular stress response in podocytes and reduces glycative and lipoperoxidative stress |
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| Verf.angabe: | Maria Scuto, Angela Trovato Salinaro, Sergio Modafferi, Alessandra Polimeni, Tilman Pfeffer, Tim Weigand, Vittorio Calabrese, Claus Peter Schmitt and Verena Peters |
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| E-Jahr: | 2020 |
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| Jahr: | 26 June 2020 |
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| Fussnoten: | Gesehen am 26.08.2020 |
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| Titel Quelle: | Enthalten in: Biomedicines |
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| Ort Quelle: | Basel : MDPI, 2013 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 8(2020,6) Artikel-Nummer 177, 14 Seiten |
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| ISSN Quelle: | 2227-9059 |
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| Abstract: | Carnosine improves diabetic complications, including diabetic nephropathy, in in vivo models. To further understand the underlying mechanism of nephroprotection, we studied the effect of carnosine under glucose-induced stress on cellular stress response proteins in murine immortalized podocytes, essential for glomerular function. High-glucose stress initiated stress response by increasing intracellular heat shock protein 70 (Hsp70), sirtuin-1 (Sirt-1), thioredoxin (Trx), glutamate-cysteine ligase (gamma-glutamyl cysteine synthetase; γ-GCS) and heme oxygenase-1 (HO-1) in podocytes by 30–50% compared to untreated cells. Carnosine (1 mM) also induced a corresponding upregulation of these intracellular stress markers, which was even more prominent compared to glucose for Hsp70 (21%), γ-GCS and HO-1 (13% and 20%, respectively; all p < 0.001). Co-incubation of carnosine (1 mM) and glucose (25 mM) induced further upregulation of Hsp70 (84%), Sirt-1 (52%), Trx (35%), γ-GCS (90%) and HO-1 (73%) concentrations compared to untreated cells (all p < 0.001). The glucose-induced increase in 4-hydroxy-trans-2-nonenal (HNE) and protein carbonylation was reduced dose-dependently by carnosine by more than 50% (p < 0.001). Although podocytes tolerated high carnosine concentrations (10 mM), high carnosine levels only slightly increased Trx and γ-GCS (10% and 19%, respectively, compared to controls; p < 0.001), but not Hsp70, Sirt-1 and HO-1 proteins (p not significant), and did not modify the glucose-induced oxidative stress response. In podocytes, carnosine induced cellular stress tolerance and resilience pathways and was highly effective in reducing high-glucose-induced glycative and lipoperoxidative stress. Carnosine in moderate concentrations exerted a direct podocyte molecular protective action. |
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| DOI: | doi:10.3390/biomedicines8060177 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/biomedicines8060177 |
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| | Volltext: https://www.mdpi.com/2227-9059/8/6/177 |
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| | DOI: https://doi.org/10.3390/biomedicines8060177 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | carnosine |
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| | diabetic nephropathy cellular stress response |
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| | glucose |
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| | oxidative stress |
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| | vitagenes |
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| K10plus-PPN: | 1727793013 |
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| Verknüpfungen: | → Zeitschrift |
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Carnosine activates cellular stress response in podocytes and reduces glycative and lipoperoxidative stress / Scuto, Maria [VerfasserIn]; 26 June 2020 (Online-Ressource)
