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| Verfasst von: | Gumiero, Andrea [VerfasserIn]  |
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| | Conz, Charlotte [VerfasserIn] |
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| | Valentín Gesé, Genís [VerfasserIn] |
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| | Zhang, Ying [VerfasserIn] |
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| | Weyer, Felix Alexander [VerfasserIn] |
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| | Lapouge, Karine [VerfasserIn] |
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| | Kappes, Julia [VerfasserIn] |
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| | von Plehwe, Ulrike [VerfasserIn] |
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| | Schermann, Géza [VerfasserIn] |
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| | Fitzke, Edith [VerfasserIn] |
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| | Wölfle, Tina [VerfasserIn] |
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| | Fischer, Tamás [VerfasserIn] |
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| | Rospert, Sabine [VerfasserIn] |
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| | Sinning, Irmgard [VerfasserIn] |
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| Titel: | Interaction of the cotranslational Hsp70 Ssb with ribosomal proteins and rRNA depends on its lid domain |
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| Verf.angabe: | Andrea Gumiero, Charlotte Conz, Genís Valentín Gesé, Ying Zhang, Felix Alexander Weyer, Karine Lapouge, Julia Kappes, Ulrike von Plehwe, Géza Schermann, Edith Fitzke, Tina Wölfle, Tamás Fischer, Sabine Rospert and Irmgard Sinning |
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| E-Jahr: | 2016 |
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| Jahr: | 24 Nov 2016 |
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| Umfang: | 12 S. |
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| Teil: | volume:7 |
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| | year:2016 |
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| | elocationid:13563 |
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| | extent:12 |
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| Fussnoten: | Gesehen am 27.08.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 7(2016), Artikel-ID 13563 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Cotranslational chaperones assist in de novo folding of nascent polypeptides in all organisms. In yeast, the heterodimeric ribosome-associated complex (RAC) forms a unique chaperone triad with the Hsp70 homologue Ssb. We report the X-ray structure of full length Ssb in the ATP-bound open conformation at 2.6 Å resolution and identify a positively charged region in the α-helical lid domain (SBDα), which is present in all members of the Ssb-subfamily of Hsp70s. Mutational analysis demonstrates that this region is strictly required for ribosome binding. Crosslinking shows that Ssb binds close to the tunnel exit via contacts with both, ribosomal proteins and rRNA, and that specific contacts can be correlated with switching between the open (ATP-bound) and closed (ADP-bound) conformation. Taken together, our data reveal how Ssb dynamics on the ribosome allows for the efficient interaction with nascent chains upon RAC-mediated activation of ATP hydrolysis. |
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| DOI: | doi:10.1038/ncomms13563 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/ncomms13563 |
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| | Volltext: https://www.nature.com/articles/ncomms13563 |
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| | DOI: https://doi.org/10.1038/ncomms13563 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 172792391X |
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| Verknüpfungen: | → Zeitschrift |
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Interaction of the cotranslational Hsp70 Ssb with ribosomal proteins and rRNA depends on its lid domain / Gumiero, Andrea [VerfasserIn]; 24 Nov 2016 (Online-Ressource)
