The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

• Verfasst von: Lauinger, Linda [VerfasserIn]
• Verfasst von: Diernfellner, Axel [VerfasserIn]
• Verfasst von: Falk, Sebastian [VerfasserIn]
• Verfasst von: Brunner, Michael [VerfasserIn]
• Titel: The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa
• Verf.angabe: Linda Lauinger, Axel Diernfellner, Sebastian Falk & Michael Brunner
• E-Jahr: 2014
• Jahr: 7 Apr 2014
• Teil: volume:5
• Teil: year:2014
• Fussnoten: Gesehen am 02.09.2020
• Titel Quelle: Enthalten in: Nature Communications
• Ort Quelle: [London] : Nature Publishing Group UK, 2010
• Jahr Quelle: 2014
• Band/Heft Quelle: 5(2014) Artikel-Nummer 3598, 10 Seiten
• ISSN Quelle: 2041-1723
• DOI: doi:10.1038/ncomms4598
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1728583020

Abstract

The Neurospora clock protein FRQ forms a complex with casein kinase 1a (CK1a) and FRH, a DEAD box-containing RNA helicase with a clock-independent essential function in RNA metabolism. In the course of a circadian period, FRQ is progressively hyperphosphorylated and eventually degraded. Timed hyperphosphorylation of FRQ is crucial for timekeeping of the clock. Here we show that the ATPase activity of FRH attenuates the kinetics of CK1a-mediated hyperphosphorylation of FRQ. Hyperphosphorylation of FRQ is strictly dependent on site-specific recruitment of a CK1a molecule that is activated upon binding.