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| Verfasst von: | Lin, Clara S. [VerfasserIn]  |
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| | Uboldi, Alessandro D. [VerfasserIn] |
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| | Marapana, Danushka [VerfasserIn] |
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| | Czabotar, Peter E. [VerfasserIn] |
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| | Epp, Christian [VerfasserIn] |
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| | Bujard, Hermann [VerfasserIn] |
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| | Taylor, Nicole L. [VerfasserIn] |
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| | Perugini, Matthew A. [VerfasserIn] |
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| | Hodder, Anthony N. [VerfasserIn] |
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| | Cowman, Alan F. [VerfasserIn] |
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| Titel: | The merozoite surface protein 1 complex Is a platform for binding to human erythrocytes by plasmodium falciparum |
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| Verf.angabe: | Clara S. Lin, Alessandro D. Uboldi, Danushka Marapana, Peter E. Czabotar, Christian Epp, Hermann Bujard, Nicole L. Taylor, Matthew A. Perugini, Anthony N. Hodder, and Alan F. Cowman |
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| E-Jahr: | 2014 |
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| Jahr: | [September 12, 2014] |
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| Umfang: | 15 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Gesehen am 03.09.2020 |
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| Titel Quelle: | Enthalten in: The journal of biological chemistry |
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| Ort Quelle: | Bethesda, Md. : Soc., 1905 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 289(2014), 37, Seite 25655-25669 |
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| ISSN Quelle: | 1083-351X |
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| Abstract: | Plasmodium falciparum is the causative agent of the most severe form of malaria in humans. The merozoite, an extracellular stage of the parasite lifecycle, invades erythrocytes in which they develop. The most abundant protein on the surface of merozoites is merozoite surface protein 1 (MSP1), which consists of four processed fragments. Studies indicate that MSP1 interacts with other peripheral merozoite surface proteins to form a large complex. Successful invasion of merozoites into host erythrocytes is dependent on this protein complex; however, the identity of all components and its function remain largely unknown. We have shown that the peripheral merozoite surface proteins MSPDBL1 and MSPDBL2 are part of the large MSP1 complex. Using surface plasmon resonance, we determined the binding affinities of MSPDBL1 and MSPDBL2 to MSP1 to be in the range of 2-4 × 10−7 m. Both proteins bound to three of the four proteolytically cleaved fragments of MSP1 (p42, p38, and p83). In addition, MSPDBL1 and MSPDBL2, but not MSP1, bound directly to human erythrocytes. This demonstrates that the MSP1 complex acts as a platform for display of MSPDBL1 and MSPDBL2 on the merozoite surface for binding to receptors on the erythrocyte and invasion. |
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| DOI: | doi:10.1074/jbc.M114.586495 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1074/jbc.M114.586495 |
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| | Volltext: http://www.jbc.org/content/289/37/25655 |
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| | DOI: https://doi.org/10.1074/jbc.M114.586495 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell Invasion |
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| | Complex |
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| | falciparum |
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| | Infection |
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| | Malaria |
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| | Merozoite |
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| | Parasite |
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| | Plasmodium |
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| | Protein Complex |
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| K10plus-PPN: | 1728768861 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ merozoite surface protein 1 complex Is a platform for binding to human erythrocytes by plasmodium falciparum / Lin, Clara S. [VerfasserIn]; [September 12, 2014] (Online-Ressource)
