Status: Bibliographieeintrag
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| Verfasst von: | Himmelreich, Nastassja [VerfasserIn]  |
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| | Kaufmann, Lilian [VerfasserIn] |
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| | Steinbeisser, Herbert [VerfasserIn] |
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| | Körner, Christian [VerfasserIn] |
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| | Thiel, Christian [VerfasserIn] |
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| Titel: | Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling |
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| Verf.angabe: | Nastassja Himmelreich, Lilian T. Kaufmann, Herbert Steinbeisser, Christian Körner, Christian Thiel |
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| E-Jahr: | 2015 |
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| Jahr: | 04 July 2015 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 03.09.2020 |
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| Titel Quelle: | Enthalten in: Journal of inherited metabolic disease |
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| Ort Quelle: | Hoboken, NJ : Wiley, 1978 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 38(2015), 6, Seite 1137-1146 |
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| ISSN Quelle: | 1573-2665 |
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| Abstract: | Reduced phosphomannomutase 2 activity in man leads to hypoglycosylation of glycoconjugates causing PMM2-CDG, the most common type of congenital disorders of glycosylation. Here we show that an antisense morpholino-mediated knockdown of the Xenopus laevis phosphomannomutase 2 gene provoked a general underglycosylation in frog embryos, which led to an altered phenotype and reduced glycosylation of Wnt5a as member of the non-canonical Wnt signalling. Loss of function experiments in hemi-sectioned embryos proved that due to the phosphomannomutase 2 knockdown expression of the Wnt5a/Ror2 target gene paraxial protocadherin was significantly decreased. Regarding the expression of paraxial protocadherin, a gain of function could only be achieved by injections of wnt5a and ror2 in dorsal neighbouring blastomeres, while a parallel injection of phosphomannomutase 2 morpholino led to a significant reduced level of expression. Our data show for the first time that a knockdown of phosphomannomutase 2 influences in vivo the non-canonical Wnt signalling during early embryogenesis. |
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| DOI: | doi:10.1007/s10545-015-9874-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s10545-015-9874-0 |
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| | Verlag: https://onlinelibrary.wiley.com/doi/abs/10.1007/s10545-015-9874-0 |
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| | DOI: https://doi.org/10.1007/s10545-015-9874-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1728776880 |
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| Verknüpfungen: | → Zeitschrift |
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Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling / Himmelreich, Nastassja [VerfasserIn]; 04 July 2015 (Online-Ressource)
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