Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Marucha, Kevin [VerfasserIn]   i
 Clayton, Christine [VerfasserIn]   i
Titel:Roles of the Pumilio domain protein PUF3 in Trypanosoma brucei growth and differentiation
Verf.angabe:K. Kamanyi Marucha and C. Clayton
E-Jahr:2020
Jahr:9 June 2020
Umfang:13 S.
Fussnoten:Gesehen am 14.09.2020
Titel Quelle:Enthalten in: Parasitology
Ort Quelle:Cambridge [u.a.] : Cambridge Univ. Press, 1908
Jahr Quelle:2020
Band/Heft Quelle:147(2020), 11, Seite 1171-1183
ISSN Quelle:1469-8161
Abstract:Trypanosomes strongly rely on post-transcriptional mechanisms to control gene expression. Several Opisthokont Pumilio domain proteins are known to suppress expression when bound to mRNAs. The Trypanosoma brucei Pumilio domain protein PUF3 is a cytosolic mRNA-binding protein that suppresses expression when tethered to a reporter mRNA. RNA-binding studies showed that PUF3 preferentially binds to mRNAs with a classical Pumilio-domain recognition motif, UGUA[U/C]AUU. RNA-interference-mediated reduction of PUF3 in bloodstream forms caused a minor growth defect, but the transcriptome was not affected. Depletion of PUF3 also slightly delayed differentiation to the procyclic form. However, both PUF3 genes could be deleted in cultured bloodstream- and procyclic-form trypanosomes. Procyclic forms without PUF3 also grew somewhat slower than wild-type, but ectopic expression of C-terminally tagged PUF3 impaired their viability. PUF3 was not required for RBP10-induced differentiation of procyclic forms to bloodstream forms. Mass spectrometry revealed no PUF3 binding partners that might explain its suppressive activity. We conclude that PUF3 may have a role in fine-tuning gene expression. Since PUF3 is conserved in all Kinetoplastids, including those that do not infect vertebrates, we suggest that it might confer advantages within the invertebrate host.
DOI:doi:10.1017/S003118202000092X
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1017/S003118202000092X
 Volltext: https://www.cambridge.org/core/journals/parasitology/article/roles-of-the-pumilio-domain-protein-puf3-in-trypanosoma-bru ...
 DOI: https://doi.org/10.1017/S003118202000092X
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:mRNA decay
 Pumilio
 translation
 Trypanosoma brucei
K10plus-PPN:1731779747
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68637043   QR-Code
zum Seitenanfang