Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| Online-Ressource |
Verfasst von: | Marucha, Kevin [VerfasserIn]  |
| Clayton, Christine [VerfasserIn]  |
Titel: | Roles of the Pumilio domain protein PUF3 in Trypanosoma brucei growth and differentiation |
Verf.angabe: | K. Kamanyi Marucha and C. Clayton |
E-Jahr: | 2020 |
Jahr: | 9 June 2020 |
Umfang: | 13 S. |
Fussnoten: | Gesehen am 14.09.2020 |
Titel Quelle: | Enthalten in: Parasitology |
Ort Quelle: | Cambridge [u.a.] : Cambridge Univ. Press, 1908 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 147(2020), 11, Seite 1171-1183 |
ISSN Quelle: | 1469-8161 |
Abstract: | Trypanosomes strongly rely on post-transcriptional mechanisms to control gene expression. Several Opisthokont Pumilio domain proteins are known to suppress expression when bound to mRNAs. The Trypanosoma brucei Pumilio domain protein PUF3 is a cytosolic mRNA-binding protein that suppresses expression when tethered to a reporter mRNA. RNA-binding studies showed that PUF3 preferentially binds to mRNAs with a classical Pumilio-domain recognition motif, UGUA[U/C]AUU. RNA-interference-mediated reduction of PUF3 in bloodstream forms caused a minor growth defect, but the transcriptome was not affected. Depletion of PUF3 also slightly delayed differentiation to the procyclic form. However, both PUF3 genes could be deleted in cultured bloodstream- and procyclic-form trypanosomes. Procyclic forms without PUF3 also grew somewhat slower than wild-type, but ectopic expression of C-terminally tagged PUF3 impaired their viability. PUF3 was not required for RBP10-induced differentiation of procyclic forms to bloodstream forms. Mass spectrometry revealed no PUF3 binding partners that might explain its suppressive activity. We conclude that PUF3 may have a role in fine-tuning gene expression. Since PUF3 is conserved in all Kinetoplastids, including those that do not infect vertebrates, we suggest that it might confer advantages within the invertebrate host. |
DOI: | doi:10.1017/S003118202000092X |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1017/S003118202000092X |
| Volltext: https://www.cambridge.org/core/journals/parasitology/article/roles-of-the-pumilio-domain-protein-puf3-in-trypanosoma-bru ... |
| DOI: https://doi.org/10.1017/S003118202000092X |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | mRNA decay |
| Pumilio |
| translation |
| Trypanosoma brucei |
K10plus-PPN: | 1731779747 |
Verknüpfungen: | → Zeitschrift |
Roles of the Pumilio domain protein PUF3 in Trypanosoma brucei growth and differentiation / Marucha, Kevin [VerfasserIn]; 9 June 2020 (Online-Ressource)
68637043