Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| |  Online-Ressource |
|---|
| Verfasst von: | Horn, Annemarie [VerfasserIn]  |
|---|
| | Hennig, Janosch [VerfasserIn] |
|---|
| | Ahmed, Yasar Luqman [VerfasserIn] |
|---|
| | Stier, Gunter [VerfasserIn] |
|---|
| | Wild, Klemens [VerfasserIn] |
|---|
| | Sattler, Michael [VerfasserIn] |
|---|
| | Sinning, Irmgard [VerfasserIn] |
|---|
| Titel: | Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction |
|---|
| Verf.angabe: | Annemarie Horn, Janosch Hennig, Yasar L. Ahmed, Gunter Stier, Klemens Wild, Michael Sattler, Irmgard Sinning |
|---|
| E-Jahr: | 2015 |
|---|
| Jahr: | 16 Nov 2015 |
|---|
| Umfang: | 11 S. |
|---|
| Teil: | volume:6 |
|---|
| | year:2015 |
|---|
| | elocationid:8875 |
|---|
| | extent:11 |
|---|
| Fussnoten: | Gesehen am 14.09.2020 |
|---|
| Titel Quelle: | Enthalten in: Nature Communications |
|---|
| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
|---|
| Jahr Quelle: | 2015 |
|---|
| Band/Heft Quelle: | 6(2015), Artikel-ID 8875 |
|---|
| ISSN Quelle: | 2041-1723 |
|---|
| Abstract: | Canonical membrane protein biogenesis requires co-translational delivery of ribosome-associated proteins to the Sec translocase and depends on the signal recognition particle (SRP) and its receptor (SR). In contrast, high-throughput delivery of abundant light-harvesting chlorophyll a,b-binding proteins (LHCPs) in chloroplasts to the Alb3 insertase occurs post-translationally via a soluble transit complex including the cpSRP43/cpSRP54 heterodimer (cpSRP). Here we describe the molecular mechanisms of tethering cpSRP to the Alb3 insertase by specific interaction of cpSRP43 chromodomain 3 with a linear motif in the Alb3 C-terminal tail. Combining NMR spectroscopy, X-ray crystallography and biochemical analyses, we dissect the structural basis for selectivity of chromodomains 2 and 3 for their respective ligands cpSRP54 and Alb3, respectively. Negative cooperativity in ligand binding can be explained by dynamics in the chromodomain interface. Our study provides a model for membrane recruitment of the transit complex and may serve as a prototype for a functional gain by the tandem arrangement of chromodomains. |
|---|
| DOI: | doi:10.1038/ncomms9875 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/ncomms9875 |
|---|
| | Volltext: https://www.nature.com/articles/ncomms9875 |
|---|
| | DOI: https://doi.org/10.1038/ncomms9875 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| K10plus-PPN: | 1731791518 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction / Horn, Annemarie [VerfasserIn]; 16 Nov 2015 (Online-Ressource)
68637071
