| |  Online-Ressource |
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| Verfasst von: | Bharat, Tanmay [VerfasserIn]  |
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| | Malsam, Jörg [VerfasserIn] |
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| | Hagen, Wim [VerfasserIn] |
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| | Malsam, Andrea [VerfasserIn] |
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| | Söllner, Thomas [VerfasserIn] |
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| | Briggs, John A. G. [VerfasserIn] |
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| Titel: | SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calcium-triggered fusion |
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| Verf.angabe: | Tanmay AM Bharat, Jörg Malsam, Wim JH Hagen, Andrea Scheutzow, Thomas H Söllner & John AG Briggs |
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| E-Jahr: | 2014 |
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| Jahr: | 3 February 2014 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 16.09.2020 |
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| Titel Quelle: | Enthalten in: European Molecular Biology OrganizationEMBO reports |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2000 |
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| Jahr Quelle: | 2014 |
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| Band/Heft Quelle: | 15(2014), 3, Seite 308-314 |
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| ISSN Quelle: | 1469-3178 |
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| Abstract: | Abstract Synaptic vesicles fuse with the plasma membrane in response to Ca2+ influx, thereby releasing neurotransmitters into the synaptic cleft. The protein machinery that mediates this process, consisting of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and regulatory proteins, is well known, but the mechanisms by which these proteins prime synaptic membranes for fusion are debated. In this study, we applied large-scale, automated cryo-electron tomography to image an in vitro system that reconstitutes synaptic fusion. Our findings suggest that upon docking and priming of vesicles for fast Ca2+-triggered fusion, SNARE proteins act in concert with regulatory proteins to induce a local protrusion in the plasma membrane, directed towards the primed vesicle. The SNAREs and regulatory proteins thereby stabilize the membrane in a high-energy state from which the activation energy for fusion is profoundly reduced, allowing synchronous and instantaneous fusion upon release of the complexin clamp. |
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| DOI: | doi:10.1002/embr.201337807 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1002/embr.201337807 |
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| | Volltext: https://www.embopress.org/doi/full/10.1002/embr.201337807 |
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| | DOI: https://doi.org/10.1002/embr.201337807 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cryo-electron microscopy |
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| | cryo-electron tomography |
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| | in vitro reconstitution |
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| | membrane curvature |
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| | SNARE-mediated membrane fusion |
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| K10plus-PPN: | 1733102086 |
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| Verknüpfungen: | → Zeitschrift |
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SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calcium-triggered fusion / Bharat, Tanmay [VerfasserIn]; 3 February 2014 (Online-Ressource)
