| |  Online-Ressource |
|---|
| Verfasst von: | Nabeta, Pamela [VerfasserIn]  |
|---|
| | Seshadri, Pratibha [VerfasserIn] |
|---|
| | Havumaki, Joshua [VerfasserIn] |
|---|
| | Mbhele, Silindile [VerfasserIn] |
|---|
| | Hendricks, Layla [VerfasserIn] |
|---|
| | Perkins, Mark D. [VerfasserIn] |
|---|
| | Nicol, Mark P. [VerfasserIn] |
|---|
| | Denkinger, Claudia M. [VerfasserIn] |
|---|
| Titel: | First clinical assessment of a prototype assay to detect the enzymatic activity of β-lactamase as a marker for pulmonary tuberculosis |
|---|
| Verf.angabe: | Pamela Nabeta, Pratibha Seshadri, Joshua Havumaki, Silindile Mbhele, Layla Hendricks, Mark D. Perkins, Mark P. Nicol, Claudia M. Denkinger |
|---|
| E-Jahr: | 2020 |
|---|
| Jahr: | 19 February 2020 |
|---|
| Umfang: | 6 S. |
|---|
| Fussnoten: | Gesehen am 18.09.2020 |
|---|
| Titel Quelle: | Enthalten in: Diagnostic microbiology and infectious disease |
|---|
| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1983 |
|---|
| Jahr Quelle: | 2020 |
|---|
| Band/Heft Quelle: | 97(2020), 2, Artikel-ID 115026, Seite 1-6 |
|---|
| ISSN Quelle: | 1879-0070 |
|---|
| Abstract: | The objective was to evaluate the sensitivity and specificity of a novel prototype test, TB REaD™, a reporter enzyme fluorescence-based assay, for pulmonary tuberculosis and to determine the optimal threshold for test positivity. This blinded, prospective study enrolled 250 patients, of which 23.2% were Mycobacterium tuberculosis complex (MTB) culture-positive. At the manufacturer-set threshold, sensitivity of the assay was 93.1% (95% confidence interval [CI] 83.3-98.1) and specificity was 8.9% (95% CI 5.2-13.8). The highest accuracy was seen at a higher threshold: sensitivity 58.6% (95% CI 44.9-71.4), specificity 59.4% (95% CI 52.1%-66.4%), with sensitivity by smear status being 40.0% (95% CI 21.1-61.3) for smear-negative and 72.7% (95% CI 54.5-86.7) for smear-positive. This study demonstrated limited accuracy of the TB REaD™ prototype for detection of pulmonary TB. Further improvements are necessary, potentially exploring probes that are more specific to MTB. |
|---|
| DOI: | doi:10.1016/j.diagmicrobio.2020.115026 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.1016/j.diagmicrobio.2020.115026 |
|---|
| | Volltext: http://www.sciencedirect.com/science/article/pii/S0732889319308181 |
|---|
| | DOI: https://doi.org/10.1016/j.diagmicrobio.2020.115026 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Diagnosis |
|---|
| | TB REaD™ |
|---|
| | Tuberculosis |
|---|
| | β-Lactamase |
|---|
| K10plus-PPN: | 1733303758 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
First clinical assessment of a prototype assay to detect the enzymatic activity of β-lactamase as a marker for pulmonary tuberculosis / Nabeta, Pamela [VerfasserIn]; 19 February 2020 (Online-Ressource)
