| Online-Ressource |
Verfasst von: | Leskelä, Jaakko [VerfasserIn]  |
| Safer, Anton [VerfasserIn]  |
| Becher, Heiko [VerfasserIn]  |
| Grau, Armin J. [VerfasserIn]  |
| Palm, Frederick [VerfasserIn]  |
Titel: | Serum lipopolysaccharide neutralizing capacity in ischemic stroke |
Verf.angabe: | Jaakko Leskelä, Milla Pietiäinen, Anton Safer, Markku Lehto, Jari Metso, Ernst Malle, Florian Buggle, Heiko Becher, Jouko Sundvall, Armin J. Grau, Pirkko J. Pussinen, Frederick Palm |
E-Jahr: | 2020 |
Jahr: | February 21, 2020 |
Umfang: | 17 S. |
Fussnoten: | Gesehen am 18.09.2020 |
Titel Quelle: | Enthalten in: PLOS ONE |
Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 15(2020,2) Artikel-Nummer e0228806, 17 Seiten |
ISSN Quelle: | 1932-6203 |
Abstract: | Introduction: Periodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizing capacity (LPS-NC) in ischemic stroke. The association of LPS-NC and Aggregatibacter actinomycetemcomitans, a major microbial biomarker in periodontitis, was also investigated. Materials and methods: The assay to measure LPS-NC was set up by spiking serum samples with E. coli LPS. The LPS-NC, LPS-binding protein (LBP), soluble CD14 (sCD14), lipoprotein profiles, apo(lipoprotein) A-I, apoB, and phospholipid transfer protein (PLTP) activity, were determined in 98 ischemic stroke patients and 100 age- and sex-matched controls. Serum and saliva immune response to A. actinomycetemcomitans, its concentration in saliva, and serotype-distribution were examined. Results: LPS-NC values ranged between 51-83% in the whole population. Although several of the LPS-NC determinants differed significantly between cases and controls (PLTP, sCD14, apoA-I, HDL-cholesterol), the levels did not (p = 0.056). The main determinants of LPS-NC were i) triglycerides (β = -0.68, p<0.001), and ii) HDL cholesterol (0.260, <0.001), LDL cholesterol (-0.265, <0.001), PLTP (-0.196, 0.011), and IgG against A. actinomycetemcomitans (0.174, 0.011). Saliva A. actinomycetemcomitans concentration was higher [log mean (95% CI), 4.39 (2.35-8.19) vs. 10.7 (5.45-21) genomes/ml, p = 0.023) and serotype D more frequent (4 vs. 0%, p = 0.043) in cases than controls. Serotypeablity or serotypes did not, however, relate to the LPS-NC. Conclusion: Serum LPS-NC comprised low PLTP-activity, triglyceride and LDL cholesterol concentrations, as well as high HDL cholesterol and IgG against A. actinomycetemcomitans. The present findings let us to conclude that LPS-NC did not associate with stroke. |
DOI: | doi:10.1371/journal.pone.0228806 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1371/journal.pone.0228806 |
| Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228806 |
| DOI: https://doi.org/10.1371/journal.pone.0228806 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Cholesterol |
| Endotoxemia |
| Endotoxins |
| Enzyme-linked immunoassays |
| Ischemic stroke |
| Lipids |
| Lipoproteins |
| Saliva |
K10plus-PPN: | 1733328068 |
Verknüpfungen: | → Zeitschrift |
Serum lipopolysaccharide neutralizing capacity in ischemic stroke / Leskelä, Jaakko [VerfasserIn]; February 21, 2020 (Online-Ressource)