Status: Bibliographieeintrag
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| Verfasst von: | Firmino, Alexandre Augusto Pereira [VerfasserIn]  |
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| | Beine-Golovchuk, Olga [VerfasserIn] |
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| Titel: | Separation and paired proteome profiling of plant chloroplast and cytoplasmic ribosomes |
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| Verf.angabe: | Alexandre Augusto Pereira Firmino, Michal Gorka, Alexander Graf, Aleksandra Skirycz, Federico Martinez-Seidel, Kerstin Zander, Joachim Kopka and Olga Beine-Golovchuk |
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| E-Jahr: | 2020 |
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| Jahr: | 14 July 2020 |
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| Umfang: | 29 S. |
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| Fussnoten: | Gesehen am 21.09.2020 |
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| Titel Quelle: | Enthalten in: Plants |
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| Ort Quelle: | Basel : MDPI, 2012 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 9(2020,7) Artikel-Nummer 892, 29 Seiten |
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| ISSN Quelle: | 2223-7747 |
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| Abstract: | Conventional preparation methods of plant ribosomes fail to resolve non-translating chloroplast or cytoplasmic ribosome subunits from translating fractions. We established preparation of these ribosome complexes from Arabidopsis thaliana leaf, root, and seed tissues by optimized sucrose density gradient centrifugation of protease protected plant extracts. The method co-purified non-translating 30S and 40S ribosome subunits separated non-translating 50S from 60S subunits, and resolved assembled monosomes from low oligomeric polysomes. Combining ribosome fractionation with microfluidic rRNA analysis and proteomics, we characterized the rRNA and ribosomal protein (RP) composition. The identity of cytoplasmic and chloroplast ribosome complexes and the presence of ribosome biogenesis factors in the 60S-80S sedimentation interval were verified. In vivo cross-linking of leaf tissue stabilized ribosome biogenesis complexes, but induced polysome run-off. Omitting cross-linking, the established paired fractionation and proteome analysis monitored relative abundances of plant chloroplast and cytoplasmic ribosome fractions and enabled analysis of RP composition and ribosome associated proteins including transiently associated biogenesis factors. |
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| DOI: | doi:10.3390/plants9070892 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/plants9070892 |
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| | Volltext: https://www.mdpi.com/2223-7747/9/7/892 |
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| | DOI: https://doi.org/10.3390/plants9070892 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | in vivo protein complex stabilization |
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| | plant proteomics |
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| | ribo-proteome |
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| | ribosome complexes |
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| | tissue specific separation |
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| K10plus-PPN: | 1733509739 |
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| Verknüpfungen: | → Zeitschrift |
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Separation and paired proteome profiling of plant chloroplast and cytoplasmic ribosomes / Firmino, Alexandre Augusto Pereira [VerfasserIn]; 14 July 2020 (Online-Ressource)
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