| |  Online-Ressource |
|---|
| Verfasst von: | Mulindwa, Julius [VerfasserIn]  |
|---|
| | Fadda, Abeer [VerfasserIn] |
|---|
| | Merce, Clementine [VerfasserIn] |
|---|
| | Matovu, Enoch [VerfasserIn] |
|---|
| | Enyaru, John [VerfasserIn] |
|---|
| | Clayton, Christine [VerfasserIn] |
|---|
| Titel: | Methods to determine the transcriptomes of trypanosomes in mixtures with mammalian cells |
|---|
| Titelzusatz: | the effects of parasite purification and selective cDNA amplification |
|---|
| Verf.angabe: | Julius Mulindwa, Abeer Fadda, Clementine Merce, Enoch Matovu, John Enyaru, Christine Clayton |
|---|
| E-Jahr: | 2014 |
|---|
| Jahr: | April 17, 2014 |
|---|
| Fussnoten: | Gesehen am 23.09.2020 |
|---|
| Titel Quelle: | Enthalten in: Public Library of SciencePLoS neglected tropical diseases |
|---|
| Ort Quelle: | Lawrence, Kan. : PLoS, 2007 |
|---|
| Jahr Quelle: | 2014 |
|---|
| Band/Heft Quelle: | 8(2014,4) Artikel-Nummer e2806, 12 Seiten |
|---|
| ISSN Quelle: | 1935-2735 |
|---|
| Abstract: | Patterns of gene expression in cultured Trypanosoma brucei bloodstream and procyclic forms have been extensively characterized, and some comparisons have been made with trypanosomes grown to high parasitaemias in laboratory rodents. We do not know, however, to what extent these transcriptomes resemble those in infected Tsetse flies - or in humans or cattle, where parasitaemias are substantially lower. For clinical and field samples it is difficult to characterize parasite gene expression because of the large excess of host cell RNA. We have here examined two potential solutions to this problem for bloodstream form trypanosomes, assaying transcriptomes by high throughput cDNA sequencing (RNASeq). |
|---|
| DOI: | doi:10.1371/journal.pntd.0002806 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pntd.0002806 |
|---|
| | Volltext: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002806 |
|---|
| | DOI: https://doi.org/10.1371/journal.pntd.0002806 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Blood |
|---|
| | cDNA synthesis |
|---|
| | Messenger RNA |
|---|
| | Parasitic diseases |
|---|
| | Red blood cells |
|---|
| | RNA sequencing |
|---|
| | Transcriptome analysis |
|---|
| | Trypanosoma |
|---|
| K10plus-PPN: | 1733627685 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Methods to determine the transcriptomes of trypanosomes in mixtures with mammalian cells / Mulindwa, Julius [VerfasserIn]; April 17, 2014 (Online-Ressource)
