| Online-Ressource |
Verfasst von: | Schröter, Philipp [VerfasserIn]  |
| Hartmann, Laura [VerfasserIn]  |
| Osen, Wolfram [VerfasserIn]  |
| Baumann, Daniel [VerfasserIn]  |
| Offringa, Rienk [VerfasserIn]  |
| Eisel, David [VerfasserIn]  |
| Debus, Jürgen [VerfasserIn]  |
| Eichmüller, Stefan B. [VerfasserIn]  |
| Rieken, Stefan [VerfasserIn]  |
Titel: | Radiation-induced alterations in immunogenicity of a murine pancreatic ductal adenocarcinoma cell line |
Verf.angabe: | Philipp Schröter, Laura Hartmann, Wolfram Osen, Daniel Baumann, Rienk Offringa, David Eisel, Jürgen Debus, Stefan B. Eichmüller, Stefan Rieken |
E-Jahr: | 2020 |
Jahr: | 20 January 2020 |
Umfang: | 10 S. |
Fussnoten: | Gesehen am 25.09.2020 |
Titel Quelle: | Enthalten in: Scientific reports |
Ort Quelle: | [London] : Springer Nature, 2011 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 10(2020) Artikel-Nummer 686, 10 Seiten |
ISSN Quelle: | 2045-2322 |
Abstract: | Pancreatic ductal adenocarcinoma (PDA) is highlighted by resistance to radiotherapy with the possible exception of hypofractionated irradiation. As single photon doses were reported to increase immunogenicity, we investigated dose-dependent irradiation effects on clonogenic survival, expression of immunologically relevant cell surface molecules and susceptibility to cytotoxic T cell (CTL) mediated killing using a murine PDA cell line. Clonogenicity decreased in a dose-responsive manner showing enhanced radioresistance at single photon doses below 5 Gy. Cell cycle analysis revealed a predominant G2/M arrest, being most pronounced 12 h after irradiation. Polyploidy increased in a dose- and time-dependent manner reaching a maximum frequency 60 h following irradiation with 10 Gy. Irradiation increased surface expression of MHC class I molecules and of immunological checkpoint molecules PDL-1 and CD73, especially at doses ≥ 5 Gy, but not of MHC class II molecules and CXCR4 receptors. Cytotoxicity assays revealed increased CTL lysis of PDA cells at doses ≥ 5 Gy. For the PDA cell line investigated, our data show for the first time that single photon doses ≥ 5 Gy effectively inhibit colony formation and induce a G2/M cell cycle arrest. Furthermore, expression levels of immunomodulatory cell surface molecules became altered possibly enhancing the susceptibility of tumour cells to CTL lysis. |
DOI: | doi:10.1038/s41598-020-57456-2 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41598-020-57456-2 |
| Volltext: https://www.nature.com/articles/s41598-020-57456-2 |
| DOI: https://doi.org/10.1038/s41598-020-57456-2 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1733724834 |
Verknüpfungen: | → Zeitschrift |
Radiation-induced alterations in immunogenicity of a murine pancreatic ductal adenocarcinoma cell line / Schröter, Philipp [VerfasserIn]; 20 January 2020 (Online-Ressource)