Stable biochemically micro-patterned hydrogel layers control specific cell adhesion and allow long term cyclic tensile strain experiments

• Verfasst von: Greiner, Alexandra M. [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Stable biochemically micro-patterned hydrogel layers control specific cell adhesion and allow long term cyclic tensile strain experiments
• Verf.angabe: Alexandra M. Greiner, Peter Hoffmann, Kristina Bruellhoff, Simon Jungbauer, Joachim P. Spatz, Martin Moeller, Ralf Kemkemer, Jürgen Groll
• E-Jahr: 2014
• Jahr: 06 August 2014
• Umfang: 9 S.
• Fussnoten: Gesehen am 29.09.2020
• Titel Quelle: Enthalten in: Macromolecular bioscience
• Ort Quelle: Weinheim : Wiley-VCH, 2001
• Jahr Quelle: 2014
• Band/Heft Quelle: 14(2014), 11, Seite 1547-1555
• ISSN Quelle: 1616-5195
• DOI: doi:10.1002/mabi.201400261
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: hydrogels
• Sach-SW: mechanical properties
• Sach-SW: micro-contact printing of fibronectin
• Sach-SW: specific cell adhesion
• Sach-SW: spin coating of star polymers
• Sach-SW: uniaxial cyclic tensile strain
• K10plus-PPN: 1733914099

Abstract

Poly(dimethylsiloxane) can be covalently coated with ultrathin NCO-sP(EO-stat-PO) hydrogel layers which permit covalent binding of cell adhesive moieties, while minimizing unspecific cell adhesion on non-functionalized areas. We applied long term uniaxial cyclic tensile strain (CTS) and revealed (a) the preservation of protein and cell-repellent properties of the NCO-sP(EO-stat-PO) coating and (b) the stability and bioactivity of a covalently bound fibronectin (FN) line pattern. We studied the adhesion of human dermal fibroblast (HDFs) on non-modified NCO-sP(EO-stat-PO) coatings and on the FN. HDFs adhered to FN and oriented their cell bodies and actin fibers along the FN lines independently of the direction of CTS. This mechanical long term stability of the bioactive, patterned surface allows unraveling biomechanical stimuli for cellular signaling and behavior to understand physiological and pathological cell phenomenon. Additionally, it allows for the application in wound healing assays, tissue engineering, and implant development demanding spatial control over specific cell adhesion.