Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| Online-Ressource |
Verfasst von: | Flemming, Dirk [VerfasserIn]  |
| Stelter, Philipp [VerfasserIn]  |
| Hurt, Ed [VerfasserIn]  |
Titel: | Utilizing the Dyn2 dimerization-zipper as a tool to probe NPC structure and function |
Verf.angabe: | Dirk Flemming, Philipp Stelter, and Ed Hurt |
E-Jahr: | 2014 |
Jahr: | 20 May 2014 |
Umfang: | 17 S. |
Fussnoten: | Gesehen am 30.09.2020 |
Titel Quelle: | Enthalten in: Nuclear pore complexes and nucleocytoplasmic transport |
Ausgabe Quelle: | First edition |
Ort Quelle: | San Diego, CA : Academic Press, 2014 |
Jahr Quelle: | 2014 |
Band/Heft Quelle: | (2014), Seite 99-115 |
ISBN Quelle: | 978-0-12-417178-7 |
| 0-12-417178-8 |
Abstract: | The discovery of dynein light chain 2 (Dyn2) as a member of the nucleoporins in yeast led to a series of applications to study NPC structure and function. Its intriguing ability to act as a hub for the parallel dimerization of two short amino acid sequence motifs (DID) prompted us to utilize it as a tool for probing nucleocytoplasmic transport in vivo. Further, the distinct structure of the Dyn2-DID rod, which is easily visible in the electron microscope, allowed us to develop a precise structural label on proteins or protein complexes. This label was used to identify the position of subunits in NPC subcomplexes or to derive at pseudo-atomic models of single large Nups. The versatility for various applications of the DID-Dyn2 system makes it an attractive molecular tool beyond the nuclear pore and transport field. |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://www.sciencedirect.com/science/article/pii/B9780124171602000059 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | DID |
| Dynein2 |
| Electron microscopy |
| FG repeats |
| Label |
| Negative stain |
| NPC |
| Transport channel |
K10plus-PPN: | 1734006781 |
Verknüpfungen: | → Sammelwerk |
Utilizing the Dyn2 dimerization-zipper as a tool to probe NPC structure and function / Flemming, Dirk [VerfasserIn]; 20 May 2014 (Online-Ressource)
68641601