| |  Online-Ressource |
|---|
| Verfasst von: | Kater, Lukas [VerfasserIn]  |
|---|
| | Mitterer, Valentin [VerfasserIn] |
|---|
| | Thoms, Matthias [VerfasserIn] |
|---|
| | Cheng, Jingdong [VerfasserIn] |
|---|
| | Berninghausen, Otto [VerfasserIn] |
|---|
| | Beckmann, Roland [VerfasserIn] |
|---|
| | Hurt, Ed [VerfasserIn] |
|---|
| Titel: | Construction of the central protuberance and L1 stalk during 60S subunit biogenesis |
|---|
| Verf.angabe: | Lukas Kater, Valentin Mitterer, Matthias Thoms, Jingdong Cheng, Otto Berninghausen, Roland Beckmann, and Ed Hurt |
|---|
| E-Jahr: | 2020 |
|---|
| Jahr: | July 14, 2020 |
|---|
| Umfang: | 19 S. |
|---|
| Titel Quelle: | Enthalten in: Molecular cell |
|---|
| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
|---|
| Jahr Quelle: | 2020 |
|---|
| Band/Heft Quelle: | 79(2020), 4, Seite 615-628.e1-e5 |
|---|
| ISSN Quelle: | 1097-4164 |
|---|
| Abstract: | Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix12-Ipi32 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression. |
|---|
| DOI: | doi:10.1016/j.molcel.2020.06.032 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.molcel.2020.06.032 |
|---|
| | DOI: https://doi.org/10.1016/j.molcel.2020.06.032 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| K10plus-PPN: | 1734619015 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Construction of the central protuberance and L1 stalk during 60S subunit biogenesis / Kater, Lukas [VerfasserIn]; July 14, 2020 (Online-Ressource)
