| |  Online-Ressource |
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| Verfasst von: | Schulze, Stefan [VerfasserIn]  |
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| | Adams, Zachary [VerfasserIn] |
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| | Cerletti, Micaela [VerfasserIn] |
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| | De Castro, Rosana [VerfasserIn] |
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| | Ferreira-Cerca, Sebastien [VerfasserIn] |
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| | Fufezan, Christian [VerfasserIn] |
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| | Gimenez, Maria Ines [VerfasserIn] |
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| | Hippler, Michael [VerfasserIn] |
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| | Jevtic, Zivojin [VerfasserIn] |
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| | Knüppel, Robert [VerfasserIn] |
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| | Legerme, Georgio [VerfasserIn] |
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| | Lenz, Christof [VerfasserIn] |
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| | Marchfelder, Anita [VerfasserIn] |
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| | Maupin-Furlow, Julie [VerfasserIn] |
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| | Paggi, Roberto A. [VerfasserIn] |
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| | Pfeiffer, Friedhelm [VerfasserIn] |
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| | Poetsch, Ansgar [VerfasserIn] |
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| | Urlaub, Henning [VerfasserIn] |
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| | Pohlschroder, Mechthild [VerfasserIn] |
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| Titel: | The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics |
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| Verf.angabe: | Stefan Schulze, Zachary Adams, Micaela Cerletti, Rosana De Castro, Sébastien Ferreira-Cerca, Christian Fufezan, María Inés Giménez, Michael Hippler, Zivojin Jevtic, Robert Knüppel, Georgio Legerme, Christof Lenz, Anita Marchfelder, Julie Maupin-Furlow, Roberto A. Paggi, Friedhelm Pfeiffer, Ansgar Poetsch, Henning Urlaub & Mechthild Pohlschroder |
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| E-Jahr: | 2020 |
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| Jahr: | 19 June 2020 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 05.10.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 11(2020) Artikel-Nummer 3145, 14 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | While many aspects of archaeal cell biology remain relatively unexplored, systems biology approaches like mass spectrometry (MS) based proteomics offer an opportunity for rapid advances. Unfortunately, the enormous amount of MS data generated often remains incompletely analyzed due to a lack of sophisticated bioinformatic tools and field-specific biological expertise for data interpretation. Here we present the initiation of the Archaeal Proteome Project (ArcPP), a community-based effort to comprehensively analyze archaeal proteomes. Starting with the model archaeon Haloferax volcanii, we reanalyze MS datasets from various strains and culture conditions. Optimized peptide spectrum matching, with strict control of false discovery rates, facilitates identifying>72% of the reference proteome, with a median protein sequence coverage of 51%. These analyses, together with expert knowledge in diverse aspects of cell biology, provide meaningful insights into processes such as N-terminal protein maturation, N-glycosylation, and metabolism. Altogether, ArcPP serves as an invaluable blueprint for comprehensive prokaryotic proteomics. While archaeal proteomics advanced rapidly, a comprehensive proteome database for archaea is lacking. Therefore, the authors here launch the Archaeal Proteome Project, a community-effort providing insights into archaeal cell biology via the combined reanalysis of Haloferax volcanii proteomics data. |
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| DOI: | doi:10.1038/s41467-020-16784-7 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-020-16784-7 |
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| | DOI: https://doi.org/10.1038/s41467-020-16784-7 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | false discovery rates |
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| | haloferax-volcanii |
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| | lonb protease |
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| | mass-spectrometry |
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| | membrane proteome |
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| | n-terminal acetylation |
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| | peptide identification |
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| | s-layer glycoprotein |
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| | signal peptides |
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| | stress responses |
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| K10plus-PPN: | 1734647248 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics / Schulze, Stefan [VerfasserIn]; 19 June 2020 (Online-Ressource)
