| |  Online-Ressource |
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| Verfasst von: | Woelfl, Franziska [VerfasserIn]  |
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| | Léger, Psylvia [VerfasserIn] |
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| | Oreshkova, Nadia [VerfasserIn] |
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| | Pahmeier, Felix [VerfasserIn] |
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| | Windhaber, Stefan [VerfasserIn] |
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| | Koch, Jana [VerfasserIn] |
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| | Stanifer, Megan [VerfasserIn] |
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| | Roman Sosa, Gleyder [VerfasserIn] |
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| | Uckeley, Zina Maria [VerfasserIn] |
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| | Rey, Felix A. [VerfasserIn] |
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| | Boulant, Steeve [VerfasserIn] |
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| | Kortekaas, Jeroen [VerfasserIn] |
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| | Wichgers Schreur, Paul J. [VerfasserIn] |
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| | Lozach, Pierre-Yves [VerfasserIn] |
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| Titel: | Novel Toscana virus reverse genetics system establishes NSs as an antagonist of type I interferon responses |
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| Verf.angabe: | Franziska Woelfl, Psylvia Léger, Nadia Oreshkova, Felix Pahmeier, Stefan Windhaber, Jana Koch, Megan Stanifer, Gleyder Roman Sosa, Zina M. Uckeley, Felix A. Rey, Steeve Boulant, Jeroen Kortekaas, Paul J. Wichgers Schreur, and Pierre-Yves Lozach |
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| E-Jahr: | 2020 |
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| Jahr: | 4 April 2020 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 08.10.2020 |
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| Titel Quelle: | Enthalten in: Viruses |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 12(2020,4) Artikel-Nummer 400, 14 Seiten |
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| ISSN Quelle: | 1999-4915 |
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| Abstract: | The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSVɸNSs). Unlike rTOSV and the wt virus, rTOSVɸNSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies. |
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| DOI: | doi:10.3390/v12040400 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/v12040400 |
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| | Volltext: https://www.mdpi.com/1999-4915/12/4/400 |
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| | DOI: https://doi.org/10.3390/v12040400 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | <i>Bunyavirales</i> |
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| | <i>Phenuiviridae</i> |
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| | <i>Phlebovirus</i> |
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| | Arbovirus |
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| | interferon |
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| | neglected diseases |
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| | reverse genetics |
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| | sand fly fever |
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| | Toscana virus |
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| K10plus-PPN: | 1735200816 |
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| Verknüpfungen: | → Zeitschrift |
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Novel Toscana virus reverse genetics system establishes NSs as an antagonist of type I interferon responses / Woelfl, Franziska [VerfasserIn]; 4 April 2020 (Online-Ressource)
