| Online-Ressource |
Verfasst von: | Bogert, Nicolai [VerfasserIn]  |
| Furkel, Jennifer [VerfasserIn]  |
| Din, Shabana [VerfasserIn]  |
| Braren, I. [VerfasserIn]  |
| Eckstein, Volker [VerfasserIn]  |
| Müller, J. A. [VerfasserIn]  |
| Uhlmann, Lorenz [VerfasserIn]  |
| Katus, Hugo [VerfasserIn]  |
| Konstandin, Mathias [VerfasserIn]  |
Titel: | A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus |
Verf.angabe: | N.V. Bogert, J. Furkel, S. Din, I. Braren, V. Eckstein, J.A. Müller, L. Uhlmann, H.A. Katus & M.H. Konstandin |
E-Jahr: | 2020 |
Jahr: | 13 August 2020 |
Umfang: | 10 S. |
Fussnoten: | Gesehen am 12.10.2020 |
Titel Quelle: | Enthalten in: Scientific reports |
Ort Quelle: | [London] : Springer Nature, 2011 |
Jahr Quelle: | 2020 |
Band/Heft Quelle: | 10(2020) Artikel-Nummer 13740, 10 Seiten |
ISSN Quelle: | 2045-2322 |
Abstract: | Lentiviral modification of hematopoietic stem cells (HSCs) paved the way for in vivo experimentation and therapeutic approaches in patients with genetic disease. A disadvantage of this method is the use of a ubiquitous promoter leads not only to genetic modification of the leukocyte subset of interest e.g. T-cells, but also all other subsequent leukocyte progeny of the parent HSCs. To overcome this limitation we tested a bicistronic lentivirus, enabling subset specific modifications. Designed novel lentiviral constructs harbor a global promoter (mPGK) regulating mCherry for HSCs selection and a T-cell specific promoter upstream of eGFP. Two T-cell specific promoters were assessed: the distal Lck—(dLck) and the CD3δ-promoter. Transduced HSCs were FACS sorted by mCherry expression and transferred into sublethally irradiated C57/BL6 mice. Successful transplantation and T-cell specific expression of eGFP was monitored by peripheral blood assessment. Furthermore, recruitment response of lentiviral engineered leukocytes to the site of inflammation was tested in a peritonitis model without functional impairment. Our constructed lentivirus enables fast generation of subset specific leukocyte transgenesis as shown in T-cells in vivo and opens new opportunities to modify other HSCs derived subsets in the future. |
DOI: | doi:10.1038/s41598-020-70793-6 |
URL: | kostenfrei: Volltext: https://doi.org/10.1038/s41598-020-70793-6 |
| kostenfrei: Volltext: https://www.nature.com/articles/s41598-020-70793-6 |
| DOI: https://doi.org/10.1038/s41598-020-70793-6 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1735364835 |
Verknüpfungen: | → Zeitschrift |
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Lokale URL UB: | Zum Volltext |
¬A¬ novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus / Bogert, Nicolai [VerfasserIn]; 13 August 2020 (Online-Ressource)