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Verfasst von:Niz, Mariana De [VerfasserIn]   i
 Kehrer, Jessica [VerfasserIn]   i
 Brancucci, Nicolas Michel Beat [VerfasserIn]   i
 Moalli, Federica [VerfasserIn]   i
 Reynaud, Emmanuel G. [VerfasserIn]   i
 Stein, Jens V. [VerfasserIn]   i
 Frischknecht, Friedrich [VerfasserIn]   i
Titel:3D imaging of undissected optically cleared Anopheles stephensi mosquitoes and midguts infected with Plasmodium parasites
Verf.angabe:Mariana De Niz, Jessica Kehrer, Nicolas M. B. Brancucci, Federica Moalli, Emmanuel G. Reynaud, Jens V. Stein, Friedrich Frischknecht
E-Jahr:2020
Jahr:September 16, 2020
Umfang:18 S.
Fussnoten:Gesehen am 22.10.2020
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2020
Band/Heft Quelle:15(2020,9) Artikel-Nummer e0238134, 18 Seiten
ISSN Quelle:1932-6203
Abstract:Malaria is a life-threatening disease, caused by Apicomplexan parasites of the Plasmodium genus. The Anopheles mosquito is necessary for the sexual replication of these parasites and for their transmission to vertebrate hosts, including humans. Imaging of the parasite within the insect vector has been attempted using multiple microscopy methods, most of which are hampered by the presence of the light scattering opaque cuticle of the mosquito. So far, most imaging of the Plasmodium mosquito stages depended on either sectioning or surgical dissection of important anatomical sites, such as the midgut and the salivary glands. Optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) enable imaging fields of view in the centimeter scale whilst providing micrometer resolution. In this paper, we compare different optical clearing protocols and present reconstructions of the whole body of Plasmodium-infected, optically cleared Anopheles stephensi mosquitoes and their midguts. The 3D-reconstructions from OPT imaging show detailed features of the mosquito anatomy and enable overall localization of parasites in midguts. Additionally, LSFM imaging of mosquito midguts shows detailed distribution of oocysts in extracted midguts. This work was submitted as a pre-print to bioRxiv, available at https://www.biorxiv.org/content/10.1101/682054v2.
DOI:doi:10.1371/journal.pone.0238134
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1371/journal.pone.0238134
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238134
 DOI: https://doi.org/10.1371/journal.pone.0238134
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Artificial light
 Imaging techniques
 Light-sheet fluorescence microscopy
 Mosquitoes
 Parasitic diseases
 Plasmodium
 Specimen sectioning
 Vision
K10plus-PPN:1736234331
Verknüpfungen:→ Zeitschrift

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