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| Verfasst von: | Burk, Alexandra Serena [VerfasserIn]  |
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| | Monzel, Cornelia [VerfasserIn] |
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| | Yoshikawa, Hiroshi [VerfasserIn] |
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| | Wuchter, Patrick [VerfasserIn] |
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| | Saffrich, Rainer [VerfasserIn] |
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| | Eckstein, Volker [VerfasserIn] |
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| | Tanaka, Motomu [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| Titel: | Quantifying adhesion Mechanisms and dynamics of human hematopoietic stem and progenitor cells |
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| Verf.angabe: | Alexandra S. Burk, Cornelia Monzel, Hiroshi Y. Yoshikawa, Patrick Wuchter, Rainer Saffrich, Volker Eckstein, Motomu Tanaka & Anthony D. Ho |
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| E-Jahr: | 2015 |
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| Jahr: | 31 March 2015 |
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| Umfang: | 8 S. |
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| Fussnoten: | Gesehen am 23.10.2020 |
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| Titel Quelle: | Enthalten in: Scientific reports |
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| Ort Quelle: | [London] : Macmillan Publishers Limited, part of Springer Nature, 2011 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 5(2015) Artikel-Nummer 9370, 8 Seiten |
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| ISSN Quelle: | 2045-2322 |
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| Abstract: | Using planar lipid membranes with precisely defined concentrations of specific ligands, we have determined the binding strength between human hematopoietic stem cells (HSC) and the bone marrow niche. The relative significance of HSC adhesion to the surrogate niche models via SDF1α-CXCR4 or N-cadherin axes was quantified by (a) the fraction of adherent cells, (b) the area of tight adhesion and (c) the critical pressure for cell detachment. We have demonstrated that the binding of HSC to the niche model is a cooperative process and the adhesion mediated by the CXCR4- SDF1α axis is stronger than that by homophilic N-cadherin binding. The statistical image analysis of stochastic morphological dynamics unraveled that HSC dissipated energy by undergoing oscillatory deformation. The combination of an in vitro niche model and novel physical tools has enabled us to quantitatively determine the relative significance of binding mechanisms between normal HSC versus leukemia blasts to the bone marrow niche. |
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| DOI: | doi:10.1038/srep09370 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/srep09370 |
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| | Volltext: https://www.nature.com/articles/srep09370 |
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| | DOI: https://doi.org/10.1038/srep09370 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1736457187 |
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| Verknüpfungen: | → Zeitschrift |
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Quantifying adhesion Mechanisms and dynamics of human hematopoietic stem and progenitor cells / Burk, Alexandra Serena [VerfasserIn]; 31 March 2015 (Online-Ressource)
