Entry of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus into the hospital

• Verfasst von: Klein, Sabrina [VerfasserIn]
• Verfasst von: Hannesen, Julius [VerfasserIn]
• Verfasst von: Zanger, Philipp [VerfasserIn]
• Verfasst von: Heeg, Klaus [VerfasserIn]
• Verfasst von: Boutin, Sébastien [VerfasserIn]
• Verfasst von: Nurjadi, Dennis [VerfasserIn]
• Titel: Entry of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus into the hospital
• Titelzusatz: prevalence and population structure in Heidelberg, Germany 2015-2018
• Verf.angabe: Sabrina Klein, Julius Hannesen, Philipp Zanger, Klaus Heeg, Sébastien Boutin & Dennis Nurjadi
• E-Jahr: 2020
• Jahr: 06 August 2020
• Umfang: 7 S.
• Fussnoten: Gesehen am 03.11.2020
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2020
• Band/Heft Quelle: 10(2020) Artikel-Nummer 13243, 7 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/s41598-020-70112-z
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1736574361
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Staphylococcus aureus is one of the major pathogens causing community—and healthcare-acquired infections. The presence of the virulence factor Panton-Valentine leukocidin (PVL) is associated with recurrent infection and clinical severity and generally regarded as a feature of community associated-methicillin-resistant Staphylococcus aureus (MRSA). To date, the focus of PVL-positive MRSA in hospitalized patients has been on outbreaks. We aimed to investigate whether PVL-positive MRSA has penetrated the community-hospital barrier by determining the prevalence of PVL in MRSA of hospitalized patients. MRSA strains isolated from patients hospitalized > 48 h in Heidelberg University Hospital between 2015 and 2018 Isolates were analysed for the presence of PVL and subjected to spa-typing. PVL-positive MRSA were then characterized by whole genome sequencing. We analysed 740 MRSA isolates in the study period and identified 6.2% (n = 46) PVL-positivity. 32.6% of PVL-positive MRSA met the criteria for nosocomial acquisition. The most frequent clones among the PVL-positive strains were ST80-t044 (21.7%, n = 10/46) and ST8-t008 (19.5%, n = 9/46). WGS identified three possible transmission clusters involving seven patients. In conclusion, we found successful epidemic PVL-positive MRSA clones entering the hospital and causing nosocomial infections. Preventive measures and constant surveillance should be maintained to prevent transmissions and clonal outbreaks.