Interaction of Cm(III) and Am(III) with human serum transferrin studied by time-resolved laser fluorescence and EXAFS spectroscopy

• Verfasst von: Bauer, Nicole [VerfasserIn]
• Verfasst von: Fröhlich, Daniel R. [VerfasserIn]
• Verfasst von: Panak, Petra [VerfasserIn]
• Titel: Interaction of Cm(III) and Am(III) with human serum transferrin studied by time-resolved laser fluorescence and EXAFS spectroscopy
• Verf.angabe: Nicole Bauer, Daniel R. Fröhlich and Petra J. Panak
• E-Jahr: 2014
• Jahr: 14 March 2014
• Umfang: 12 S.
• Fussnoten: Gesehen am 26.10.2020
• Titel Quelle: Enthalten in: Dalton transactions
• Ort Quelle: London : Soc., 1972
• Jahr Quelle: 2014
• Band/Heft Quelle: 43(2014), 18, Seite 6689-6700
• ISSN Quelle: 1477-9234
• DOI: doi:10.1039/C3DT53371A
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1736574604

Abstract

The complexation of Cm(III) with human serum transferrin was investigated in a pH range from 3.5 to 11.0 using time-resolved laser fluorescence spectroscopy (TRLFS). At pH ≥ 7.4 Cm(III) is incorporated at the Fe(III) binding site of transferrin whereas at lower pH a partially bound Cm(III) transferrin species is formed. At physiological temperature (310 K) at pH 7.4, about 70% of the partially bound and 30% of the incorporated Cm(III) transferrin species are present in solution. The Cm(III) results obtained by TRLFS are in very good agreement with Am(III) EXAFS results, confirming the incorporation of Am(III) at the Fe(III) binding site at pH 8.5.