Critical view on RNA silencing-mediated virus resistance using exogenously applied RNA

• Verfasst von: Uslu, Veli Vural [VerfasserIn]
• Verfasst von: Wassenegger, Michael [VerfasserIn]
• Titel: Critical view on RNA silencing-mediated virus resistance using exogenously applied RNA
• Verf.angabe: Veli V Uslu and Michael Wassenegger
• E-Jahr: 2020
• Jahr: 1st May 2020
• Umfang: 7 S.
• Fussnoten: Gesehen am 26.10.2020
• Titel Quelle: Enthalten in: Current opinion in virology
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 2011
• Jahr Quelle: 2020
• Band/Heft Quelle: 42(2020), Seite 18-24
• ISSN Quelle: 1879-6265
• DOI: doi:10.1016/j.coviro.2020.03.004
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1736576887

Abstract

In almost all eukaryotes, RNA interference (RNAi) is a natural defence mechanism against foreign nucleic acids, including transposons and viruses. It is generally triggered by long double stranded RNA molecules (dsRNA, >50bp) that are processed into small interfering RNAs (siRNAs). RNAi can be artificially activated by the expression of RNAi triggers through viruses (virus-induced gene silencing, VIGS) and transgenes. Moreover, for almost 10 years, exogenous RNA application methods are developed as tools to induce RNAi in plants. In this review, exogenous RNA application techniques having the potential to activate RNAi with a focus on RNAi-mediated virus resistance will be discussed. Limitations of exogenous RNA applications, targeting of virus vectors and open questions related to mechanistic details that still require further investigation will be pointed out.