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| Verfasst von: | Dao Thi, Viet Loan [VerfasserIn]  |
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| | Wu, Xianfang [VerfasserIn] |
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| | Belote, Rachel L. [VerfasserIn] |
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| | Andreo, Ursula [VerfasserIn] |
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| | Takacs, Constantin N. [VerfasserIn] |
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| | Fernandez, Joseph P. [VerfasserIn] |
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| | Vale Silva, Luis A. [VerfasserIn] |
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| | Prallet, Sarah [VerfasserIn] |
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| | Decker, Charlotte Caroline [VerfasserIn] |
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| | Fu, Rebecca Menhua [VerfasserIn] |
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| | Qu, Bingqian [VerfasserIn] |
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| | Uryu, Kunihiro [VerfasserIn] |
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| | Molina, Henrik [VerfasserIn] |
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| | Saeed, Mohsan [VerfasserIn] |
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| | Steinmann, Eike [VerfasserIn] |
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| | Urban, Stephan [VerfasserIn] |
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| | Singaraja, Roshni R. [VerfasserIn] |
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| | Schneider, William M. [VerfasserIn] |
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| | Simon, Sanford M. [VerfasserIn] |
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| | Rice, Charles M. [VerfasserIn] |
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| Titel: | Stem cell-derived polarized hepatocytes |
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| Verf.angabe: | Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon & Charles M. Rice |
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| E-Jahr: | 2020 |
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| Jahr: | 03 April 2020 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 29.10.2020 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 11(2020) Artikel-Nummer 1677, 13 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell differentiation protocol that uses transwell filters to generate columnar polarized HLCs with clearly defined basolateral and apical membranes separated by tight junctions. We show that polarized HLCs secrete cargo directionally: Albumin, urea, and lipoproteins are secreted basolaterally, whereas bile acids are secreted apically. Further, we show that enterically transmitted hepatitis E virus (HEV) progeny particles are secreted basolaterally as quasi-enveloped particles and apically as naked virions, recapitulating essential steps of the natural infectious cycle in vivo. We also provide proof-of-concept that polarized HLCs can be used for pharmacokinetic and drug-drug interaction studies. This novel system provides a powerful tool to study hepatocyte biology, disease mechanisms, genetic variation, and drug metabolism in a more physiologically relevant setting. |
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| DOI: | doi:10.1038/s41467-020-15337-2 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-020-15337-2 |
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| | Volltext: https://www.nature.com/articles/s41467-020-15337-2 |
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| | DOI: https://doi.org/10.1038/s41467-020-15337-2 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1737357542 |
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| Verknüpfungen: | → Zeitschrift |
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Stem cell-derived polarized hepatocytes / Dao Thi, Viet Loan [VerfasserIn]; 03 April 2020 (Online-Ressource)
