| |  Online-Ressource |
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| Verfasst von: | Pei, Weike [VerfasserIn]  |
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| | Shang, Fuwei [VerfasserIn] |
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| | Wang, Xi [VerfasserIn] |
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| | Fanti, Ann-Kathrin [VerfasserIn] |
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| | Greco, Alessandro [VerfasserIn] |
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| | Busch, Katrin [VerfasserIn] |
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| | Klapproth, Kay [VerfasserIn] |
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| | Zhang, Qin [VerfasserIn] |
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| | Quedenau, Claudia [VerfasserIn] |
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| | Sauer, Sascha [VerfasserIn] |
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| | Feyerabend, Thorsten B. [VerfasserIn] |
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| | Höfer, Thomas [VerfasserIn] |
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| | Rodewald, Hans-Reimer [VerfasserIn] |
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| Titel: | Resolving fates and single-cell transcriptomes of hematopoietic stem cell clones by PolyloxExpress barcoding |
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| Verf.angabe: | Weike Pei, Fuwei Shang, Xi Wang, Ann-Kathrin Fanti, Alessandro Greco, Katrin Busch, Kay Klapproth, Qin Zhang, Claudia Quedenau, Sascha Sauer, Thorsten B. Feyerabend, Thomas Höfer, Hans-Reimer Rodewald |
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| E-Jahr: | 2020 |
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| Jahr: | 11 August 2020 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 29.10.2020 |
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| Titel Quelle: | Enthalten in: Cell stem cell |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 2007 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 27(2020), 3, Seite 383-395.e8 |
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| ISSN Quelle: | 1875-9777 |
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| Abstract: | Lineage tracing reveals hematopoietic stem cell (HSC) fates, while single-cell RNA sequencing identifies snapshots of HSC transcriptomes. To obtain information on fate plus transcriptome in the same cell, we developed the PolyloxExpress allele, enabling Cre-recombinase-dependent RNA barcoding in situ. Linking fates to single HSC transcriptomes provided the information required to identify transcriptional signatures of HSC fates, which were not apparent in single-HSC transcriptomes alone. We find that differentiation-inactive, multilineage, and lineage-restricted HSC clones reside in distinct regions of the transcriptional landscape of hematopoiesis. Differentiation-inactive HSC clones are closer to the origin of the transcriptional trajectory, yet they are not characterized by a quiescent gene signature. Fate-specific gene signatures imply coherence of clonal HSC fates, and HSC output toward short-lived lineage progenitors indicates stability of HSC fates over time. These combined analyses of fate and transcriptome under physiological conditions may pave the way toward identifying molecular determinants of HSC fates. |
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| DOI: | doi:10.1016/j.stem.2020.07.018 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.stem.2020.07.018 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S1934590920303568 |
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| | DOI: https://doi.org/10.1016/j.stem.2020.07.018 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | clonal fate coherence |
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| | hematopoietic stem cell fates |
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| | hematopoietic stem cell quiescence |
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| | hematopoietic stem cells |
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| | high-resolution RNA barcoding |
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| | lineage tracing |
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| | single-cell transcriptomics |
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| | transcriptional landscape |
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| K10plus-PPN: | 1737390701 |
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| Verknüpfungen: | → Zeitschrift |
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Resolving fates and single-cell transcriptomes of hematopoietic stem cell clones by PolyloxExpress barcoding / Pei, Weike [VerfasserIn]; 11 August 2020 (Online-Ressource)
