| |  Online-Ressource |
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| Verfasst von: | Kounatidis, Ilias [VerfasserIn]  |
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| | Stanifer, Megan [VerfasserIn] |
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| | Phillips, Michael A. [VerfasserIn] |
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| | Paul-Gilloteaux, Perrine [VerfasserIn] |
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| | Heiligenstein, Xavier [VerfasserIn] |
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| | Wang, Hongchang [VerfasserIn] |
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| | Okolo, Chidinma A. [VerfasserIn] |
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| | Fish, Thomas M. [VerfasserIn] |
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| | Spink, Matthew C. [VerfasserIn] |
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| | Stuart, David I. [VerfasserIn] |
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| | Davis, Ilan [VerfasserIn] |
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| | Boulant, Steeve [VerfasserIn] |
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| | Grimes, Jonathan M. [VerfasserIn] |
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| | Dobbie, Ian M. [VerfasserIn] |
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| | Harkiolaki, Maria [VerfasserIn] |
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| Titel: | 3D correlative cryo-structured illumination fluorescence and soft X-ray microscopy elucidates reovirus intracellular release pathway |
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| Verf.angabe: | Ilias Kounatidis, Megan L. Stanifer, Michael A. Phillips, Perrine Paul-Gilloteaux, Xavier Heiligenstein, Hongchang Wang, Chidinma A. Okolo, Thomas M. Fish, Matthew C. Spink, David I. Stuart, Ilan Davis, Steeve Boulant, Jonathan M. Grimes, Ian M. Dobbie, and Maria Harkiolaki |
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| E-Jahr: | 2020 |
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| Jahr: | 23 July 2020 |
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| Umfang: | 34 S. |
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| Fussnoten: | Gesehen am 29.10.2020 |
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| Titel Quelle: | Enthalten in: Cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1974 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 182(2020), 2, Seite 515-530.e17 |
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| ISSN Quelle: | 1097-4172 |
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| Abstract: | Imaging of biological matter across resolution scales entails the challenge of preserving the direct and unambiguous correlation of subject features from the macroscopic to the microscopic level. Here, we present a correlative imaging platform developed specifically for imaging cells in 3D under cryogenic conditions by using X-rays and visible light. Rapid cryo-preservation of biological specimens is the current gold standard in sample preparation for ultrastructural analysis in X-ray imaging. However, cryogenic fluorescence localization methods are, in their majority, diffraction-limited and fail to deliver matching resolution. We addressed this technological gap by developing an integrated, user-friendly platform for 3D correlative imaging of cells in vitreous ice by using super-resolution structured illumination microscopy in conjunction with soft X-ray tomography. The power of this approach is demonstrated by studying the process of reovirus release from intracellular vesicles during the early stages of infection and identifying intracellular virus-induced structures. |
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| DOI: | doi:10.1016/j.cell.2020.05.051 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.cell.2020.05.051 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S009286742030684X |
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| | DOI: https://doi.org/10.1016/j.cell.2020.05.051 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CLXT |
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| | correlative imaging |
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| | reovirus biology |
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| | structured illumination microscopy |
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| | X-ray tomography |
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| K10plus-PPN: | 1737393328 |
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| Verknüpfungen: | → Zeitschrift |
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3D correlative cryo-structured illumination fluorescence and soft X-ray microscopy elucidates reovirus intracellular release pathway / Kounatidis, Ilias [VerfasserIn]; 23 July 2020 (Online-Ressource)
